Yokomuro Hiroki, Shiono Noritsugu, Sasaki Yuki, Katayanagi Tomoyuki, Hara Masanori, Yoshihara Katsunori, Watanabe Yoshinori
Division of Cardiovascular Surgery, Department of Surgery, School of Medicine, Faculty of Medicine, Toho University, Tokyo, Japan.
Ann Thorac Cardiovasc Surg. 2013;19(2):107-12. doi: 10.5761/atcs.oa.11.01876. Epub 2012 Aug 31.
To determine whether a dynamic cultured biograft can positively affect the function of the damaged heart.
We ligated the coronary artery (LAD) of rats to generate a model of myocardial infarction (MI) and then implanted them with the following grafts comprising vascular smooth muscle cells (VSMCs) derived from the rat aorta and seeded onto biodegradable patches (patch replacement therapy; (PRTx)): control without PRTx, PRTx without seeded cells, PRTx with static cultured VSMCs, PRTx with dynamic cultured VSMCs and sham-operated. Cultured VSMCs were labeled with PKH26 for identification after implantation, and the centre of the MI site was excised and replaced with an implanted biograft. Cardiac performance was monitored for 12 weeks thereafter and followed by a histological study.
Although the ejection fraction of the damaged heart improved in all groups that were transplanted with grafts, remodeling was prevented only in groups with a dynamic or static cultured patch. More cells were α-SMA-positive in the group with the dynamic, rather than the static cultured patch. Cells were positive for PKH26 in the biograft and in the infarcted myocardium.
Dynamic cultured biografts improved the function of the infarcted myocardium more than statically cultured biografts or those without cells.
确定动态培养的生物移植物是否能对受损心脏的功能产生积极影响。
我们结扎大鼠冠状动脉（左前降支）以建立心肌梗死（MI）模型，然后给它们植入以下移植物，这些移植物包含源自大鼠主动脉的血管平滑肌细胞（VSMC）并接种到可生物降解的贴片上（贴片置换疗法；（PRTx））：无PRTx的对照组、无接种细胞的PRTx、静态培养VSMC的PRTx、动态培养VSMC的PRTx以及假手术组。植入后，用PKH26标记培养的VSMC以进行识别，切除MI部位的中心并用植入的生物移植物替换。此后监测心脏功能12周，然后进行组织学研究。
尽管所有移植移植物的组中受损心脏的射血分数均有所改善，但仅动态或静态培养贴片组的心脏重塑得到了预防。动态培养贴片组中α-SMA阳性的细胞比静态培养贴片组更多。生物移植物和梗死心肌中的细胞PKH26呈阳性。
与静态培养的生物移植物或无细胞的生物移植物相比，动态培养的生物移植物更能改善梗死心肌的功能。
