Service d'Urologie et Chirurgie de la Transplantation, Université Lyon 1, Lyon, France.
Prostate. 2012 May 15;72(7):713-20. doi: 10.1002/pros.21473. Epub 2011 Aug 31.
A number of putative stem cell markers have been associated with aggressiveness of prostate cancer, including alpha 2 and alpha 6 integrin and c-met. The study aimed to test the hypothesis that the development of bone metastasis correlates with the proportion of prostate cancer stem cell-like cells present in the primary tumor.
Prostate tissue samples were obtained from patients with high-risk prostatic adenocarcinoma. Prostate cancer tumor tissue samples underwent immunohistochemical staining for alpha 2 and alpha 6 integrin and c-met; positive and negative controls were included. Samples were scored as positive if >5% of cells within the sample stained positively. Survival and bone metastasis-free survival curves on the patient cohort were estimated by the actuarial method of Kaplan-Meier.
A total of 62 patients were included in the study. Bone metastases progression rate was 46% at 105 months with a median time of 46 months (95% CI: 1-62.5 months); prostate cancer-specific survival was 33% at 122 months with a median survival time of 69.4 months (95% CI: 63.5-109.4 months). Survival curves show that c-met-, alpha 2, and alpha 6 integrin-positive tumors were positively associated with the occurrence of bone metastasis-free survival. There was a higher level of significance when at least c-met and either alpha 2 or alpha 6 integrin was positive.
It can be concluded that percentage of stem cell-like prostate cancer cells has a prognostic impact especially on the risk of metastatic bone progression.
一些推测的干细胞标志物与前列腺癌的侵袭性有关，包括α2 和α6 整合素和 c-met。本研究旨在检验这样一个假设，即骨转移的发展与原发性肿瘤中存在的前列腺癌干细胞样细胞的比例相关。
从高危前列腺腺癌患者中获得前列腺组织样本。对前列腺癌肿瘤组织样本进行α2 和α6 整合素和 c-met 的免疫组织化学染色；包括阳性和阴性对照。如果样本中>5%的细胞染色阳性，则将样本评为阳性。通过 Kaplan-Meier 的累积法估计患者队列的生存和无骨转移生存曲线。
共有 62 例患者纳入研究。骨转移进展率为 105 个月时为 46%，中位时间为 46 个月（95%CI：1-62.5 个月）；前列腺癌特异性生存率为 122 个月时为 33%，中位生存时间为 69.4 个月（95%CI：63.5-109.4 个月）。生存曲线表明，c-met、α2 和α6 整合素阳性肿瘤与无骨转移生存的发生呈正相关。当至少 c-met 和α2 或α6 整合素之一阳性时，其显著性水平更高。
可以得出结论，具有干细胞样特性的前列腺癌细胞的比例对特别是转移性骨进展的风险具有预后影响。
