RNA 动力学：自旋标记的视角。
RNA dynamics: perspectives from spin labels.
机构信息
Department of Chemistry, University of Southern California, Los Angeles, CA, USA.
出版信息
Wiley Interdiscip Rev RNA. 2012 Jan-Feb;3(1):62-72. doi: 10.1002/wrna.104. Epub 2011 Aug 22.
Abstract
Dynamics are important and indispensible physical attributes that play essential roles in RNA function. RNA dynamics are complex, spanning vast timescales, and encompassing a large number of physical modes. The technique of site-directed spin labeling (SDSL), which derives information on local structural and dynamic features of a macromolecule by monitoring a chemically stable nitroxide radical using electron paramagnetic resonance spectroscopy, has been applied to monitor intrinsic dynamics at defined structural states as well as to probe conformational transition dynamics of RNAs. The current state of SDSL studies of RNA dynamics is summarized here. Further development and application of SDSL promise to open up many more opportunities for probing RNA dynamics and connecting dynamics to structure and function.
摘要
动力学是重要且不可或缺的物理属性，在 RNA 功能中起着至关重要的作用。RNA 动力学复杂，跨越巨大的时间尺度，包含大量物理模式。通过使用电子顺磁共振波谱学监测化学稳定的氮氧自由基，来获取大分子局部结构和动态特征信息的定点自旋标记（SDSL）技术已被应用于监测定义结构状态下的固有动力学，以及探测 RNA 的构象转变动力学。本文总结了 SDSL 研究 RNA 动力学的现状。SDSL 的进一步发展和应用有望为探测 RNA 动力学并将动力学与结构和功能联系起来提供更多机会。
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