Zhang Qing-Li, Wu Qian, Xie Yuan-Yuan, Shen Lian-Zhong, Fan Min-Wei, Liang Qiong-Lin, Wang Yi-Ming, Luo Guo-An
Analysis Center, Department of Chemistry, Tsinghua University, Beijing 100084, China.
Yao Xue Xue Bao. 2011 Jun;46(6):701-6.
This study is to report the tissue distribution of arsenic after giving different doses of realgar and Liushen pills to Beagle dogs, in order to provide basis for the safety evaluation of Liushen pills. ICP-MS was used to measure arsenic concentration, and HPLC-ICP-MS was used to analyze arsenic speciation. The concentration of total arsenic and As(III) + DMA (arsenite + dimethylarsenic acid) increased with dosing of realgar. Total arsenic concentration in most tissues and As(III) + DMA concentration in all tissues of Liushen pills group are lower than that of realgar group, but AsB concentration in liver, spleen and kidney of Liushen pills group increased. The concentration of total arsenic showed a dose-dependent manner with dosage administered. It was indicated that components in Liushen pills can reduce solubility of arsenic in realgar, which may decrease toxicity of realgar.
本研究旨在报告给比格犬给予不同剂量雄黄和六神丸后砷的组织分布情况，为六神丸的安全性评价提供依据。采用电感耦合等离子体质谱法（ICP-MS）测定砷浓度，采用高效液相色谱-电感耦合等离子体质谱法（HPLC-ICP-MS）分析砷形态。随着雄黄给药量增加，总砷浓度以及As(III)+DMA（亚砷酸盐+二甲基砷酸）浓度升高。六神丸组大多数组织中的总砷浓度以及所有组织中的As(III)+DMA浓度均低于雄黄组，但六神丸组肝脏、脾脏和肾脏中的AsB浓度升高。总砷浓度随给药剂量呈剂量依赖性。表明六神丸中的成分可降低雄黄中砷的溶解度，这可能会降低雄黄的毒性。
