Zhang Yun-jing, Qiang Shu-ping, Song Min, Hang Tai-jun
Yao Xue Xue Bao. 2016 Jul;51(7):1130-5.
The arsenic species in rat plasma were studied after oral administration of realgar and Niu Huang Jie Du Pian (NHJDP) and the possible compatible effects of realgar was evaluated by comparing the pharmacokinetics of arsenic species after administration of realgar and NHJDP. The separation of the arsenicals was performed by a high performance liquid chromatography-hydride generation-atomic fluorescence spectrometry (HPLC-HG-AFS) technique. Dimethylarsinic acid (DMA) was found to be the main species in rats’ plasma after dosing. No traces of arsenite [As(Ⅲ)], monomethylarsonic acid (MMA) or arsenate [As(Ⅴ)] were detected at any sampling time points. Compared with realgar administration alone, dose-normalized peak concentration(C(max)) and AUC(0-t) of DMA were significantly decreased by NHJDP administration, while the t(max) was significantly delayed with the clearance and apparent volume of distribution significantly increased, indicating that the pharmacokinetics of As from realgar was affected by other ingredients in the compound prescription of NHJDP.
在大鼠口服雄黄和牛黄解毒片（NHJDP）后，对大鼠血浆中的砷形态进行了研究，并通过比较雄黄和NHJDP给药后砷形态的药代动力学，评估了雄黄可能的配伍效应。采用高效液相色谱-氢化物发生-原子荧光光谱法（HPLC-HG-AFS）对砷化物进行分离。结果发现，给药后大鼠血浆中的主要砷形态为二甲基砷酸（DMA）。在任何采样时间点均未检测到亚砷酸盐[As(Ⅲ)]、一甲基砷酸（MMA）或砷酸盐[As(Ⅴ)]。与单独给予雄黄相比，NHJDP给药后，DMA的剂量归一化峰浓度（C(max)）和AUC(0-t)显著降低，而t(max)显著延迟，清除率和表观分布容积显著增加，表明NHJDP复方制剂中的其他成分影响了雄黄中砷的药代动力学。
