刺孢毛霉对小豆蔻明的微生物糖基化作用。

Microbial glycosylation of cardamonin by Mucor spinosus.

作者信息

Xu Jing, Yang Li, Zhao Shu-Juan, Chou Gui-Xin, Wang Zheng-Tao

机构信息

Department of Pharmacognosy, China Pharmaceutical University, Nanjing 210038, China.

出版信息

Yao Xue Xue Bao. 2011 Jun;46(6):733-7.

PMID:21882537

Abstract

Microbial transformation of cardamonin by Mucor spinosus (CGMCC 3.3450) in preparative scale resulted in the isolation of two new products. Their structures were elucidated unambiguously by ESI-MS, 1H NMR, 13C NMR and 2D NMR spectra analyses as 4-O-beta-D-glucopyranosyl-6-hydroxy-2-methoxychalcone (1, 4-GluC) and 6-O-beta-D-glucopyranosyl-4-hydroxy-2-methoxychalcone (2, 6-GluC), respectively. The time-course of biotransformation by M. spinosus showed that both 4-GluC and 6-GluC appeared on the 2nd day. The optimal biotransformation temperature was 28 degrees C, the optimal biotransformation time was 72 h and the optimal concentration for cardamonin was 40 mg x mL(-1). This is the first time for successful microbial glycosylation of cardamonin in present research.

摘要

在制备规模下，棘孢毛霉（CGMCC 3.3450）对小豆蔻明进行微生物转化，得到了两种新产物。通过电喷雾电离质谱（ESI-MS）、氢核磁共振谱（1H NMR）、碳核磁共振谱（13C NMR）和二维核磁共振谱分析，分别明确鉴定出它们的结构为4-O-β-D-吡喃葡萄糖基-6-羟基-2-甲氧基查耳酮（1, 4-GluC）和6-O-β-D-吡喃葡萄糖基-4-羟基-2-甲氧基查耳酮（2, 6-GluC）。棘孢毛霉的生物转化时间进程表明，4-GluC和6-GluC均在第2天出现。最佳生物转化温度为28℃，最佳生物转化时间为72小时，小豆蔻明的最佳浓度为40 mg·mL-1。在本研究中，这是首次成功实现小豆蔻明的微生物糖基化。