Department of Entomology, University College of Agriculture, Bahauddin Zakariya University, Multan, Pakistan.
J Econ Entomol. 2011 Aug;104(4):1343-8. doi: 10.1603/ec10383.
Two field populations of Spodoptera exigua (Hübner) (Lepidoptera: Noctuidae) from Dera Ghazi Khan (D. G. Khan) and Multan, Pakistan, were tested for resistance to the 10 most commonly used insecticides in Pakistan by using a standard leaf disc bioassay on the F1 progeny. For comparison, a susceptible strain was generated from the Multan strain, which displayed lower LC50 values for most of the insecticides, by either mass rearing without exposure to insecticides or single-pair crosses against selected insecticides. The single-pair crosses generated a more susceptible strain than mass rearing. The D. G. Khan field strain was highly resistant to cypermethrin, profenofos, spinosad, abamectin, and chlorpyrifos and moderately resistant to deltamethrin, indoxacarb, and methoxyfenozide compared with susceptible lab strain. The Multan strain was highly resistant to profenofos and indoxacarb. Both field populations were susceptible to emamectin benzoate and lufenuron. Rotating these two insecticides with others that show very low, low, or moderate levels of resistance and have different modes of action may be useful for the effective management of this pest.
来自巴基斯坦德拉加济汗（Dera Ghazi Khan）和木尔坦的 2 个草地夜蛾（Spodoptera exigua）种群（鳞翅目：夜蛾科）通过对 F1 后代进行标准叶片圆盘生物测定，对巴基斯坦最常用的 10 种杀虫剂进行了抗药性测试。为了进行比较，从对大多数杀虫剂具有较低 LC50 值的木尔坦种群中，通过不接触杀虫剂的大量繁殖或针对选定杀虫剂的单对杂交，产生了一个敏感品系。单对杂交产生的品系比大量繁殖更为敏感。与敏感的实验室品系相比，DG 汗野外种群对氯氰菊酯、丙溴磷、多杀菌素、阿维菌素和毒死蜱高度耐药，对溴氰菊酯、茚虫威和甲氧虫酰肼中度耐药。木尔坦种群对丙溴磷和茚虫威高度耐药。这两个田间种群对甲氨基阿维菌素苯甲酸盐和灭蝇脲敏感。与那些表现出非常低、低或中度水平抗性且具有不同作用模式的其他杀虫剂轮换使用，可能有助于有效防治这种害虫。
