Division of Nanobiomedicine, Suzhou Institute of Nano-tech and Nano-bionics, Chinese Academy of Sciences, 398 Ruoshui Road, Suzhou 215123, China.
ACS Appl Mater Interfaces. 2011 Oct;3(10):4085-91. doi: 10.1021/am2009647. Epub 2011 Sep 19.
Formation of composites of dextran-coated Fe(3)O(4) nanoparticles (NPs) and graphene oxide (Fe(3)O(4)-GO) and their application as T(2)-weighted contrast agent for efficient cellular magnetic resonance imaging (MRI) are reported. Aminodextran (AMD) was first synthesized by coupling reaction of carboxymethyldextran with butanediamine, which was then chemically conjugated to meso-2,3-dimercaptosuccinnic acid-modified Fe(3)O(4) NPs. Next, the AMD-coated Fe(3)O(4) NPs were anchored onto GO sheets via formation of amide bond in the presence of 1-ethyl-3-(3-dimethyaminopropyl) carbodiimide (EDC). It is found that the Fe(3)O(4)-GO composites possess good physiological stability and low cytotoxicity. Prussian Blue staining analysis indicates that the Fe(3)O(4)-GO nanocomposites can be internalized efficiently by HeLa cells, depending on the concentration of the composites incubated with the cells. Furthermore, compared with the isolated Fe(3)O(4) NPs, the Fe(3)O(4)-GO composites show significantly enhanced cellular MRI, being capable of detecting cells at the iron concentration of 5 μg mL(-1) with cell density of 2 × 10(5) cells mL(-1), and at the iron concentration of 20 μg mL(-1) with cell density of 1000 cells mL(-1).
报道了葡聚糖包覆的 Fe(3)O(4) 纳米粒子（NPs）和氧化石墨烯（Fe(3)O(4)-GO）复合材料的形成及其作为 T(2)加权对比剂在高效细胞磁共振成像（MRI）中的应用。首先通过羧甲基葡聚糖与丁二胺的偶联反应合成了氨丙基葡聚糖（AMD），然后通过在 1-乙基-3-（3-二甲基氨基丙基）碳二亚胺（EDC）的存在下形成酰胺键将其化学接枝到间-2,3-二巯基丁二酸修饰的 Fe(3)O(4) NPs 上。结果发现，Fe(3)O(4)-GO 复合材料具有良好的生理稳定性和低细胞毒性。普鲁士蓝染色分析表明，Fe(3)O(4)-GO 纳米复合材料可以通过 HeLa 细胞有效内化，这取决于与细胞共孵育的复合材料的浓度。此外，与分离的 Fe(3)O(4) NPs 相比，Fe(3)O(4)-GO 复合材料显示出明显增强的细胞 MRI，能够在铁浓度为 5μg mL(-1)、细胞密度为 2×10(5)个细胞 mL(-1)的条件下检测到细胞，在铁浓度为 20μg mL(-1)、细胞密度为 1000 个细胞 mL(-1)的条件下检测到细胞。
