Zhou Chaohui, Wu Hui, Wang Mingliang, Huang Chusen, Yang Dapeng, Jia Nengqin
The Education Ministry Key Laboratory of Resource Chemistry, Shanghai Key Laboratory of Rare Earth Functional Materials and Shanghai Municipal Education Committee Key Laboratory of Molecular Imaging Probes and Sensors, Department of Chemistry, Shanghai Normal University, Shanghai 200234, China.
Department of Radiology, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
Mater Sci Eng C Mater Biol Appl. 2017 Sep 1;78:817-825. doi: 10.1016/j.msec.2017.04.139. Epub 2017 Apr 23.
In this work, we developed a T-weighted contrast agent based on graphene oxide (GO)/FeO hybrids for efficient cellular magnetic resonance imaging (MRI). The GO/FeO hybrids were obtained by combining with co-precipitation method and pyrolysis method. The structural, surface and magnetic characteristics of the hybrids were systematically characterized by transmission electron microscopy (TEM), vibrating sample magnetometer (VSM), AFM, Raman, FT-IR and XRD. The GO/FeO hybrids were functionalized by modifying with anionic and cationic polyelectrolyte through layer-by-layer assembling. The fluorescence probe fluorescein isothiocyanate (FITC) was further loaded on the surface of functionalized GO/FeO hybrids to trace the location of GO/FeO hybrids in cells. Functionalized GO/FeO hybrids possess good hydrophilicity, less cytotoxicity, high MRI enhancement with the relaxivity (r) of 493mMs as well as cellular MRI contrast effect. These obtained results indicated that the functionalized GO/FeO hybrids could have great potential to be utilized as cellular MRI contrast agents for tumor early diagnosis and monitoring.
在本研究中，我们基于氧化石墨烯（GO）/FeO杂化物开发了一种T加权造影剂，用于高效的细胞磁共振成像（MRI）。通过共沉淀法和热解法相结合获得了GO/FeO杂化物。利用透射电子显微镜（TEM）、振动样品磁强计（VSM）、原子力显微镜（AFM）、拉曼光谱、傅里叶变换红外光谱（FT-IR）和X射线衍射（XRD）对杂化物的结构、表面和磁性特征进行了系统表征。通过层层组装用阴离子和阳离子聚电解质对GO/FeO杂化物进行功能化修饰。进一步将荧光探针异硫氰酸荧光素（FITC）负载在功能化的GO/FeO杂化物表面，以追踪GO/FeO杂化物在细胞中的位置。功能化的GO/FeO杂化物具有良好的亲水性、较低的细胞毒性、高MRI增强效果，弛豫率（r）为493 mM⁻¹s⁻¹，以及细胞MRI造影效果。这些结果表明，功能化的GO/FeO杂化物在用作肿瘤早期诊断和监测的细胞MRI造影剂方面具有巨大潜力。
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