Chiang Tzen-Yuh, Lee Teh-Wang, Hsu Kui-Ching, Kuo Chien-Hsien, Lin Der-Yuh, Lin Hung-Du
Department of Life Sciences, National Cheng-Kung University, Tainan, 701, Taiwan.
Zoolog Sci. 2011 Sep;28(9):642-51. doi: 10.2108/zsj.28.642.
The genetic structure of four populations of Pararasbora moltrechti, an endemic species of the Cyprinidae in Taiwan, was investigated based on the genetic variation of mtDNA Cyt b gene and five microsatellite loci. High haplotype diversity (h = 0.92) but low nucleotide diversity (0.004) in mtDNA was detected in this endangered species. In total, 33 haplotypes and four clusters were identified in its mtDNA. Nevertheless, low correspondence was found between geographical division and mtDNA clusters. In contrast, Bayesian cluster analysis of the microsatellite data identified four genetic groups and revealed highly structured populations. Significantly negative Tajima's D statistics and mismatch distribution analyses suggest that P. moltrechti populations may have experienced a demographic expansion. In light of the results of a nested clade analysis of mtDNA haplotypes, we conclude that recent population fluctuations and restricted gene flow played major roles in shaping the spatial genetic structure of P. moltrechti populations.
基于线粒体DNA细胞色素b基因的遗传变异和五个微卫星位点，对台湾鲤科特有物种莫氏副细鲫四个种群的遗传结构进行了研究。在这种濒危物种中，检测到线粒体DNA的单倍型多样性较高（h = 0.92），但核苷酸多样性较低（0.004）。其线粒体DNA总共鉴定出33个单倍型和四个聚类。然而，地理划分与线粒体DNA聚类之间的对应性较低。相比之下，微卫星数据的贝叶斯聚类分析确定了四个遗传组，并揭示了种群的高度结构化。显著负的Tajima's D统计量和失配分布分析表明，莫氏副细鲫种群可能经历了种群扩张。根据线粒体DNA单倍型的嵌套分支分析结果，我们得出结论，近期的种群波动和有限的基因流在塑造莫氏副细鲫种群的空间遗传结构中起主要作用。
