Kuo Hsiao-Che, Hsu Hao-Hsuan, Chua Chee Shin, Wang Ting-Yu, Chen Young-Mao, Chen Tzong-Yueh
Laboratory of Molecular Genetics, Institute of Biotechnology, College of Bioscience and Biotechnology, National Cheng Kung University, Tainan 70101, Taiwan.
Mar Drugs. 2014 Apr 30;12(5):2397-407. doi: 10.3390/md12052397.
Most giant groupers in the market are derived from inbred stock. Inbreeding can cause trait depression, compromising the animals' fitness and disease resistance, obligating farmers to apply increased amounts of drugs. In order to solve this problem, a pedigree classification method is needed. Here, microsatellite and mitochondrial DNA were used as genetic markers to analyze the genetic relationships among giant grouper broodstocks. The 776-bp fragment of high polymorphic mitochondrial D-loop sequence was selected for measuring sibling relatedness. In a sample of 118 giant groupers, 42 haplotypes were categorized, with nucleotide diversity (π) of 0.00773 and haplotype diversity (HD) of 0.983. Furthermore, microsatellites were used for investigation of parentage. Six out of 33 microsatellite loci were selected as markers based on having a high number of alleles and compliance with Hardy-Weinberg equilibrium. Microsatellite profiles based on these loci provide high variability with low combined non-exclusion probability, permitting practical use in aquaculture. The method described here could be used to improve grouper broodstock management and lower the chances of inbreeding. This approach is expected to lead to production of higher quality groupers with higher disease resistance, thereby reducing the need for drug application.
市场上的大多数巨石斑鱼都来自近亲繁殖的种群。近亲繁殖会导致性状衰退，损害动物的健康和抗病能力，迫使养殖者增加药物用量。为了解决这个问题，需要一种谱系分类方法。在这里，微卫星和线粒体DNA被用作遗传标记，以分析巨石斑鱼亲鱼之间的遗传关系。选择了高多态性线粒体D环序列的776bp片段来测量同胞亲缘关系。在118条巨石斑鱼的样本中，分类出42种单倍型，核苷酸多样性（π）为0.00773，单倍型多样性（HD）为0.983。此外，微卫星被用于亲子鉴定研究。基于等位基因数量多且符合哈迪-温伯格平衡，从33个微卫星位点中选择了6个作为标记。基于这些位点的微卫星图谱具有高变异性和低联合非排除概率，可在水产养殖中实际应用。这里描述的方法可用于改善石斑鱼亲鱼管理，降低近亲繁殖的几率。这种方法有望培育出抗病能力更强、品质更高的石斑鱼，从而减少药物使用需求。
