Luz V B, Santos Rr, Araújo V R, Celestino J J H, Magalhães-Padilha D M, Chaves R N, Brito I R, Silva T F P, Almeida A P, Campello C C, Figueiredo J R
Faculty of Veterinary Medicine, LAMOFOPA, PPGCV, State University of Ceará, Fortaleza, Brazil.
Reprod Domest Anim. 2012 Jun;47(3):379-84. doi: 10.1111/j.1439-0531.2011.01883.x. Epub 2011 Aug 30.
We investigated the effect of the leukaemia inhibitory factor (LIF) alone or in association with FSH on the in vitro culture (IVC) of caprine preantral follicles. Preantral follicles >200 μm in size were isolated and cultured for 18 days in basic medium either alone (control) or supplemented with LIF (10 or 50 ng/ml) in the absence or presence of FSH. Every 6 days, follicular survival, growth and antrum formation were evaluated. At the end of the culture period, the oocytes underwent in vitro maturation (IVM), and their viability and chromatin configuration were assessed. Follicles of the control group and those cultured in 10 ng/ml LIF maintained the structural integrity (particularly the preservation of the basement membrane) when compared to the oocytes cultured in 50 ng/ml LIF, regardless the presence of FSH. In the absence of FSH, the percentage of antrum formation after 18 days of culture in the 50 ng/ml LIF group was significantly lower than in either the control group or the 10 ng/ml LIF group. However, this effect was not observed in the presence of FSH. The rate of resumption of meiosis was significantly higher in the 50 ng/ml LIF group in the absence of FSH in comparison with the control and 10 ng/ml LIF groups. Metaphase II was observed only when follicles were cultured in a combination of FSH and 50 ng/ml LIF. In conclusion, LIF alone does not interfere with antral formation and oocyte growth, but at concentration of 50 ng/ml and combined with FSH, it promotes oocyte maturation.
我们研究了白血病抑制因子（LIF）单独或与促卵泡激素（FSH）联合使用对山羊腔前卵泡体外培养（IVC）的影响。分离出直径大于200μm的腔前卵泡，在基础培养基中单独培养（对照组）或在不存在或存在FSH的情况下添加LIF（10或50 ng/ml）培养18天。每6天评估卵泡的存活、生长和腔形成情况。培养期结束时，卵母细胞进行体外成熟（IVM），并评估其活力和染色质构型。与在50 ng/ml LIF中培养的卵母细胞相比，对照组和在10 ng/ml LIF中培养的卵泡保持了结构完整性（特别是基底膜的保存），无论是否存在FSH。在不存在FSH的情况下，50 ng/ml LIF组培养18天后的腔形成百分比显著低于对照组或10 ng/ml LIF组。然而，在存在FSH的情况下未观察到这种效应。与对照组和10 ng/ml LIF组相比，在不存在FSH的情况下，50 ng/ml LIF组减数分裂恢复率显著更高。仅当卵泡在FSH和50 ng/ml LIF组合中培养时才观察到中期II。总之，单独的LIF不干扰腔形成和卵母细胞生长，但在50 ng/ml浓度并与FSH联合使用时，它促进卵母细胞成熟。
