del Rio Gomez I, Bratthall D
Department of Cariology, School of Dentistry, University of Lund, Malmö, Sweden.
Scand J Dent Res. 1990 Apr;98(2):106-11. doi: 10.1111/j.1600-0722.1990.tb00948.x.
According to a new method for sampling mutans streptococci, "Strip mutans", a plastic strip is contaminated by human saliva and incubated in a selective broth. After growth the strip with adherent colonies may be dried and stored. We wanted to know for how long a time bacteria in the colonies were viable if the strips were stored under different conditions. The survival time is important if the bacteria need to be subjected to further analyses. The results showed that the viability of the bacteria after the incubation period was at least 1 yr when the strips were stored frozen or frozen in glycerine. Stored dried in room temperature the survival was about 8 wk. A second part of the study aimed at identifying the colonies on strips obtained from different countries. Dried strips were sent air mail to our laboratory for biochemical and immunological analyses and the results showed that serotype/biotype c/e/f of S. mutans were most frequent but d/g S. sobrinus was also present in the samples, which came from Brazil, Greece, and Sweden. Three colonies of 213 on these strips did not grow, possibly indicating non-mutans bacteria.
根据一种用于采样变形链球菌的新方法“变形链球菌条带法”，将一条塑料条带被人唾液污染后，置于选择性肉汤中培养。生长后，带有附着菌落的条带可以干燥并储存。我们想知道，如果条带在不同条件下储存，菌落中的细菌能存活多长时间。如果需要对细菌进行进一步分析，存活时间很重要。结果表明，当条带冷冻或在甘油中冷冻保存时，培养期后细菌的活力至少为1年。在室温下干燥保存，存活时间约为8周。该研究的第二部分旨在鉴定从不同国家获得的条带上的菌落。干燥的条带通过航空邮件寄到我们实验室进行生化和免疫学分析，结果表明，变形链球菌血清型/生物型c/e/f最为常见，但样品中也存在d/g型表兄链球菌，这些样品来自巴西、希腊和瑞典。这些条带上213个菌落中有3个没有生长，这可能表明存在非变形链球菌细菌。
