Jensen B, Bratthall D
Department of Cariology, School of Denistry, University of Lund, Malmö, Sweden.
J Dent Res. 1989 Mar;68(3):468-71. doi: 10.1177/00220345890680030601.
A new method for quantitating the numbers of mutans streptococci in saliva--the "Strip mutans" test--was developed. It includes the following steps: A prepared plastic spatula is contaminated with saliva, transferred to a selective broth, incubated for two days, and dried. A predetermined area is counted or evaluated for CFUs of mutans streptococci. The medium contains sucrose to promote adhesion of mutans streptococci to the strip. Bacitracin, added just before use, is the basis for the selectivity. Results with the new medium were compared with those from the "spatula technique" and with those from conventional saliva sampling with dilution. The studies involved 302 and 60 schoolchildren, respectively. There was a highly significant correlation between the results from the new method and those from conventional methods. The strip mutans test is easy to used and has long shelf-life, and the spatulas with adherent colonies can be saved for future comparisons.
一种用于定量唾液中变形链球菌数量的新方法——“变形链球菌试纸”试验被开发出来。它包括以下步骤：将一个已准备好的塑料刮铲用唾液污染，转移至选择性肉汤中，培养两天，然后干燥。对变形链球菌的菌落形成单位（CFU）计数或评估一个预定区域。该培养基含有蔗糖以促进变形链球菌附着于试纸上。使用前刚添加的杆菌肽是选择性的基础。将新培养基的结果与“刮铲技术”的结果以及常规唾液稀释取样的结果进行了比较。这些研究分别涉及302名和60名学童。新方法的结果与常规方法的结果之间存在高度显著的相关性。变形链球菌试纸试验易于使用且保质期长，带有附着菌落的刮铲可以保存以备将来比较。
