Department of Bacteriology, Tuberculosis Research Centre (Indian Council of Medical Research), Mayor V. R. Ramanathan Road,Chetput, Chennai, Tamil Nadu, India.
Clin Microbiol Infect. 2012 May;18(5):497-501. doi: 10.1111/j.1469-0691.2011.03601.x. Epub 2011 Aug 29.
Phage lysin was evaluated as a substitute for antibiotics in sputum samples processed by a modified Petroff's method for the detection of Mycobacterium tuberculosis with the MGIT 960 system. One hundred and fifty sputum samples were processed, inoculated onto two slopes of Lowenstein-Jensen medium, and divided in to two aliquots of 0.5 mL each. One aliquot was added to 7 mL of MGIT medium containing polymyxin B, amphotericin B, nalidixic acid, trimethoprim and azlocillin (PANTA) (MGIT-PANTA) and the other was added to 7 mL of MGIT medium containing 0.8 mL of lysin (MGIT-Lysin). The samples were randomized and incubated at 37°C in the MGIT 960 system. The sensitivity and specificity of MGIT-Lysin were 97% and 88%, respectively, as compared with MGIT-PANTA. The average times to detection with MGIT-Lysin and MGIT-PANTA were 9.3 and 8.6 days, respectively. The rate of contamination with MGIT-PANTA and MGIT-Lysin were 16% and 7.3%, respectively. Phage lysin can be substituted for antibiotics in processed sputum samples for the detection of M. tuberculosis.
噬菌体溶素被评估为替代抗生素，用于改良 Petroff 法处理的痰标本，通过 MGIT 960 系统检测结核分枝杆菌。处理了 150 份痰标本，接种到两个 Lowenstein-Jensen 培养基斜面上，并分为两个 0.5mL 的等分试样。一份加入含有多粘菌素 B、两性霉素 B、萘啶酸、甲氧苄啶和氨曲南的 7mL MGIT 培养基（MGIT-PANTA），另一份加入含有 0.8mL 溶素的 7mL MGIT 培养基（MGIT-Lysin）。样本随机分组，在 MGIT 960 系统中 37°C 孵育。与 MGIT-PANTA 相比，MGIT-Lysin 的灵敏度和特异性分别为 97%和 88%。使用 MGIT-Lysin 和 MGIT-PANTA 的平均检测时间分别为 9.3 和 8.6 天。MGIT-PANTA 和 MGIT-Lysin 的污染率分别为 16%和 7.3%。噬菌体溶素可替代抗生素，用于处理痰标本以检测结核分枝杆菌。
