Department of Medical Biochemistry, Faculty of Medicine, Hacettepe University, Ankara, Turkey.
Int J Lab Hematol. 2012 Apr;34(2):129-35. doi: 10.1111/j.1751-553X.2011.01369.x. Epub 2011 Aug 24.
Sickle-cell anemia (SCA) is a disease of high oxidative stress. The oxidative medium of SCA was evaluated by protein oxidation parameters and their correlation with lipids and ions were investigated both in the plasma and in the erythrocyte.
Blood samples were obtained from 15 steady-state patients with SCA, 10 carriers, and 10 controls. Plasma protein carbonyl, total sulfhydryl, total cholesterol, triglyceride, phospholipids, and bilirubin levels were determined by spectrophotometric methods. Plasma copper, zinc, and iron levels were determined by atomic absorption spectrophotometry. Levels of cholesterol, triglyceride, and ions were also determined in the hemolysate lipid extract.
Patients had increased protein carbonyl and decreased total sulfhydryl levels compared with controls. Plasma cholesterol levels were lower than controls, and triglyceride levels were higher than carriers in patients with SCA. Plasma iron and hemolysate copper were higher in patients than carriers and controls. Plasma protein carbonyl levels were found to be correlated with plasma iron and hemolysate zinc levels in the patient group.
Alterations of proteins, lipids, and ions in the plasma and erythrocyte of steady-state patients with SCA were demonstrated. Some of these alterations are related with each other and with the oxidative stress observed in the disease.
镰状细胞贫血（SCA）是一种氧化应激水平较高的疾病。本研究通过蛋白氧化参数评估 SCA 的氧化环境，并调查了其与血浆和红细胞中脂质和离子的相关性。
采集 15 例稳定期 SCA 患者、10 例 SCA 携带者和 10 例健康对照者的血液样本。采用分光光度法测定血浆蛋白羰基、总巯基、总胆固醇、甘油三酯、磷脂和胆红素水平。采用原子吸收分光光度法测定血浆铜、锌和铁水平。还测定了溶血脂质提取物中的胆固醇、甘油三酯和离子水平。
与对照组相比，患者的蛋白羰基水平升高，总巯基水平降低。SCA 患者的血浆胆固醇水平低于对照组，甘油三酯水平高于携带者。与携带者和对照组相比，患者的血浆铁和溶血铜水平更高。在患者组中发现，血浆蛋白羰基水平与血浆铁和溶血锌水平相关。
本研究证明了稳定期 SCA 患者的血浆和红细胞中的蛋白质、脂质和离子发生了改变。其中一些改变与疾病中观察到的氧化应激相关，且彼此之间也存在关联。
