Canellas C G L, Carvalho S M F, Anjos M J, Lopes R T
Nuclear Instrumentation Laboratory, COPPE/UFRJ, P.O. Box 68509, 21.941-972 Rio de Janeiro, Brazil.
Appl Radiat Isot. 2012 Jul;70(7):1277-80. doi: 10.1016/j.apradiso.2011.12.028. Epub 2012 Feb 15.
In this work we analyzed serum samples from patients with Sickle Cell Anemia (SCA) using Total Reflection X-Ray Fluorescence using Synchrotron Radiation (SRTXRF). The SRTXRF measurements were performed at the X-Ray Fluorescence Beamline at the Brazilian National Synchrotron Light Laboratory (LNLS). We studied forty-three patients aged 18-50 suffering from SCA and sixty healthy volunteers aged 18-60. It was possible to determine the concentrations of the following elements: P, S, Cl, K, Ca, Fe, Cu, Zn, Br and Rb. Moreover, there are evidences of an association among Fe, Cu, Zn and Cu/Zn in the SCA pathogenesis process. The concentrations of Fe and Cu in the serum samples of patients with SCA were larger, 120% and 20%, respectively, when compared with the CG. The serum level Cu/Zn ratio was significantly higher (60%) in the serum samples from patients suffering from SCA than from the CG. Therefore, the Cu/Zn ratio can be used as an adjuvant index in enhancement for diagnosis of SCA.
在这项工作中，我们使用同步辐射全反射X射线荧光光谱法（SRTXRF）分析了镰状细胞贫血（SCA）患者的血清样本。SRTXRF测量在巴西国家同步辐射光实验室（LNLS）的X射线荧光光束线上进行。我们研究了43名年龄在18至50岁之间的SCA患者和60名年龄在18至60岁之间的健康志愿者。可以确定以下元素的浓度：磷、硫、氯、钾、钙、铁、铜、锌、溴和铷。此外，有证据表明铁、铜、锌以及铜/锌之间在SCA发病过程中存在关联。与对照组相比，SCA患者血清样本中铁和铜的浓度分别更高，分别为120%和20%。SCA患者血清样本中的血清铜/锌比值显著高于对照组（60%）。因此，铜/锌比值可作为辅助指标用于增强SCA的诊断。
