Faculty of Pharmaceutical Sciences, Tokyo University of Science, 2641 Yamazaki Noda Chiba 278-8510, Japan.
Colloids Surf B Biointerfaces. 2011 Dec 1;88(2):706-10. doi: 10.1016/j.colsurfb.2011.08.004. Epub 2011 Aug 12.
Nanoparticles effectively deliver therapeutic agent by penetrating into the skin. Indomethacin (IM) and coumarin-6 were loaded in PLGA nanoparticles with an average diameter of 100 nm. IM and coumarin-6 were chosen as a model drug and as a fluorescent marker, respectively. The surfaces of the nanoparticles were negatively charged. Permeability of IM-loaded PLGA nanoparticles through rat skin was studied. Higher amount of IM was delivered through skin when IM was loaded in nanoparticles than IM was free molecules. Also, iontophoresis was applied to enhance the permeability of nanoparticles. When iontophoresis with 3 V/cm was applied, permeability of IM was much higher than that obtained by simple diffusion of nanoparticles through skin. The combination of charged nanoparticle system with iontophoresis is useful for effective transdermal delivery of therapeutic agents.
纳米粒子通过穿透皮肤有效地递送治疗剂。将吲哚美辛（IM）和香豆素-6 载入平均直径为 100nm 的 PLGA 纳米粒子中。IM 和香豆素-6 分别被选择为模型药物和荧光标记物。纳米粒子的表面带负电荷。研究了载有 IM 的 PLGA 纳米粒子通过大鼠皮肤的渗透情况。当 IM 载入纳米粒子中时，通过皮肤传递的 IM 量比 IM 自由分子多。此外，还应用离子电渗法来增强纳米粒子的渗透性。当施加 3V/cm 的离子电渗时，IM 的渗透性比通过皮肤简单扩散的纳米粒子的渗透性高得多。带电荷的纳米粒子系统与离子电渗法的结合对于治疗剂的经皮有效递送是有用的。
