Lehrstuhl für Molekularbiologie der Pflanzen (Botanik), Department Biologie I, Ludwig-Maximilians-Universität München, Großhaderner Str. 2, D-82152 Planegg-Martinsried, Germany.
Curr Genomics. 2011 Mar;12(1):1-14. doi: 10.2174/138920211794520187.
Since the sequencing of the nuclear genome of Arabidopsis thaliana ten years ago, various large-scale analyses of gene function have been performed in this model species. In particular, the availability of collections of lines harbouring random T-DNA or transposon insertions, which include mutants for almost all of the ~27,000 A. thaliana genes, has been crucial for the success of forward and reverse genetic approaches. In the foreseeable future, genome-wide phenotypic data from mutant analyses will become available for Arabidopsis, and will stimulate a flood of novel in-depth gene-function analyses. In this review, we consider the present status of resources and concepts for systematic studies of gene function in A. thaliana. Current perspectives on the utility of loss-of-function and gain-of-function mutants will be discussed in light of the genetic and functional redundancy of many A. thaliana genes.
自十年前拟南芥核基因组测序以来，在这个模式物种中进行了各种大规模的基因功能分析。特别是，含有随机 T-DNA 或转座子插入的品系收集的可用性，其中包括几乎所有~27000 个拟南芥基因的突变体，对于正向和反向遗传学方法的成功至关重要。在可预见的将来，拟南芥突变体分析的全基因组表型数据将可用，并将激发大量新的深入的基因功能分析。在这篇综述中，我们考虑了拟南芥中系统研究基因功能的资源和概念的现状。将根据许多拟南芥基因的遗传和功能冗余性来讨论功能丧失和功能获得突变体的效用的当前观点。
