缺乏多巴胺和/或 5-羟色胺转运体的小鼠中 MDMA 对细胞外多巴胺和 5-羟色胺水平的影响。
Effects of MDMA on Extracellular Dopamine and Serotonin Levels in Mice Lacking Dopamine and/or Serotonin Transporters.
机构信息
Division of Psychobiology, Tokyo Institute of Psychiatry, Tokyo 156-8585, Japan.
出版信息
Curr Neuropharmacol. 2011 Mar;9(1):91-5. doi: 10.2174/157015911795017254.
3,4-Methylendioxymethamphetamine (MDMA) has both stimulatory and hallucinogenic properties which make its psychoactive effects unique and different from those of typical psychostimulant and hallucinogenic agents. The present study investigated the effects of MDMA on extracellular dopamine (DA(ex)) and serotonin (5-HT(ex)) levels in the striatum and prefrontal cortex (PFC) using in vivo microdialysis techniques in mice lacking DA transporters (DAT) and/or 5-HT transporters (SERT). subcutaneous injection of MDMA (3, 10 mg/kg) significantly increased striatal DA(ex) in wild-type mice, SERT knockout mice, and DAT knockout mice, but not in DAT/SERT double-knockout mice. The MDMA-induced increase in striatal DA(ex) in SERT knockout mice was significantly less than in wildtype mice. In the PFC, MDMA dose-dependently increased DA(ex) levels in wildtype, DAT knockout, SERT knockout and DAT/SERT double-knockout mice to a similar extent. In contrast, MDMA markedly increased 5-HT(ex) in wildtype and DAT knockout mice and slightly increased 5-HT(ex) in SERT-KO and DAT/SERT double-knockout mice. The results confirm that MDMA acts at both DAT and SERT and increases DA(ex) and 5-HT(ex).
3,4-亚甲二氧基甲基苯丙胺（MDMA）具有刺激和致幻特性，使其精神活性作用与典型的精神兴奋剂和致幻剂不同。本研究使用体内微透析技术，在缺乏多巴胺转运体（DAT）和/或 5-羟色胺转运体（SERT）的小鼠中，研究了 MDMA 对纹状体和前额叶皮层（PFC）细胞外多巴胺（DA（ex））和 5-羟色胺（5-HT（ex））水平的影响。皮下注射 MDMA（3、10mg/kg）显著增加野生型小鼠、SERT 敲除小鼠和 DAT 敲除小鼠的纹状体 DA（ex），但不增加 DAT/SERT 双敲除小鼠的纹状体 DA（ex）。SERT 敲除小鼠纹状体 DA（ex）的 MDMA 诱导增加明显少于野生型小鼠。在 PFC 中，MDMA 剂量依赖性地增加野生型、DAT 敲除、SERT 敲除和 DAT/SERT 双敲除小鼠的 DA（ex）水平，增加幅度相似。相比之下，MDMA 显著增加野生型和 DAT 敲除小鼠的 5-HT（ex），并轻微增加 SERT-KO 和 DAT/SERT 双敲除小鼠的 5-HT（ex）。结果证实 MDMA 作用于 DAT 和 SERT，并增加 DA（ex）和 5-HT（ex）。
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