Division of Psychobiology, Tokyo Institute of Psychiatry, Tokyo 156-8585, Japan.
Curr Neuropharmacol. 2011 Mar;9(1):219-22. doi: 10.2174/157015911795016921.
Increasing evidence suggests that µ opioid receptor (MOP) expression is altered during the development of and withdrawal from substance dependence. Although anti-MOP antibodies have been hypothesized to be useful for estimating MOP expression levels, inconsistent MOP molecular weights (MWs) have been reported in studies using anti-MOP antibodies. In the present study, we generated a new anti-MOP antibody (N38) against the 1-38 amino acid sequence of the mouse MOP N-terminus and conducted Western blot analysis with wildtype and MOP knockout brain lysates to determine the MWs of intrinsic MOP. The N38 antibody detected migrating bands with relative MWs of 60-67 kDa in the plasma membrane fraction isolated from wildtype brain, but not from the MOP knockout brain. These migrating bands exhibited semi-linear density in the range of 3-30 µg membrane proteins/lane. The N38 antibody may be useful for quantitatively detecting MOP.
越来越多的证据表明，μ阿片受体（MOP）的表达在物质依赖的发展和戒断过程中发生改变。尽管抗 MOP 抗体被假设可用于估计 MOP 表达水平，但在使用抗 MOP 抗体的研究中，报道的 MOP 分子量（MW）并不一致。在本研究中，我们针对小鼠 MOP N 端的 1-38 个氨基酸序列生成了一种新的抗 MOP 抗体（N38），并使用野生型和 MOP 敲除脑裂解物进行 Western blot 分析，以确定内源性 MOP 的 MW。N38 抗体在从野生型大脑中分离的质膜部分中检测到相对 MW 为 60-67 kDa 的迁移带，但在 MOP 敲除大脑中未检测到。这些迁移带在 3-30 µg 膜蛋白/泳道范围内呈半线性密度。N38 抗体可能有助于定量检测 MOP。
