BioOptics Group, Rosario Institute of Physics (CONICET-UNR), Boulevard 27 de Febrero 210 bis, Rosario 2000, Argentina.
J Biomed Mater Res A. 2011 Dec 1;99(3):445-54. doi: 10.1002/jbm.a.33193. Epub 2011 Sep 1.
To mask the antigenic sites of cells for cell therapies, especially for blood transfusion, we investigated the hemocompatibility of two poly(2-(dimethylamino)ethyl methacrylate-co-poly(ethyleneglycol) compared with that of the homopolymer without PEG. Our strategy relies on the potential ability of these copolymers to self-assemble at the erythrocyte surface. The cationic sequence of the copolymer should be able to interact with the glycocalyx by ionic interaction. The other sequence, based on a polyethyleneglycol moiety, should prevent both nonspecific interactions and specific recognition of the biological surface. The hemocompatibility of these copolymers was assessed by analyzing alterations in human erythrocyte membrane viscoelasticity, morphology, granularity, and aggregation. Their properties to mask ABO system and three erythrocyte glycophorin sites were investigated. No alterations in the erythrocyte morphology were observed by confocal microscopy. On the other hand, a partial masking of different specific glycophorin sites leads to future optimization of the macromolecular structures of these functionalized copolymers.
为了掩蔽细胞的抗原性部位用于细胞治疗，特别是用于输血，我们研究了两种聚（2-（二甲氨基）乙基甲基丙烯酸酯-co-聚（乙二醇）与无 PEG 的均聚物相比的血液相容性。我们的策略依赖于这些共聚物在红细胞表面自组装的潜在能力。共聚物的阳离子序列应该能够通过离子相互作用与糖萼相互作用。另一个序列基于聚乙二醇部分，应该防止非特异性相互作用和生物表面的特异性识别。通过分析人红细胞膜粘弹性、形态、粒度和聚集的变化来评估这些共聚物的血液相容性。还研究了它们掩蔽 ABO 系统和三种红细胞糖蛋白部位的特性。共聚焦显微镜观察到红细胞形态没有变化。另一方面，不同特定糖蛋白部位的部分掩蔽导致这些功能化共聚物的大分子结构的进一步优化。
