Ishihara K, Tsuji T, Kurosaki T, Nakabayashi N
Institute for Medical and Dental Engineering, Tokyo Medical and Dental University, Japan.
J Biomed Mater Res. 1994 Feb;28(2):225-32. doi: 10.1002/jbm.820280213.
To improve the hemocompatibility on hydrophobic biomedical materials by a simple coating technique, graft copolymers composed of a hydrophilic side chain with phospholipid polar groups and a hydrophobic backbone were synthesized. The hydrophilic chain had phospholipid polar groups, poly[2-methacryloyloxyethyl phosphorylcholine (MPC)], and the hydrophobic backbone was poly[n-butyl methacrylate (BMA)]. Because the graft copolymers obtained could dissolve in ethanol, they could be used as a coating material. When the poly(MPC-graft-BMA) was coated onto a poly(BMA) membrane, the composition of the MPC units on the surface was maintained in the bulk graft copolymer even after immersion in water. Protein adsorption on the membrane coated with the graft copolymer from human plasma detected by a gold-colloid labeled immunoassay was drastically decreased compared with that on glass and the original membrane. Moreover, blood cell adhesion, activation, and aggregation on the membrane after contact with human citrated whole blood were suppressed by the coating of the graft copolymer. These results clearly show that the poly(MPC-graft-BMA) is a suitable material for improving hemocompatibility on the biomedical devices because of its protein adsorption and cell adhesion resistant properties.
为了通过一种简单的涂层技术提高疏水性生物医学材料的血液相容性，合成了由带有磷脂极性基团的亲水性侧链和疏水性主链组成的接枝共聚物。亲水性链含有磷脂极性基团聚[2-甲基丙烯酰氧基乙基磷酰胆碱（MPC）]，疏水性主链是聚甲基丙烯酸正丁酯（BMA）。由于得到的接枝共聚物可溶于乙醇，它们可用作涂层材料。当聚（MPC-接枝-BMA）涂覆在聚（BMA）膜上时，即使浸入水中后，表面上MPC单元的组成仍保持在本体接枝共聚物中。通过金胶体标记免疫测定法检测，与人血浆接触后，接枝共聚物涂覆的膜上的蛋白质吸附与玻璃和原始膜相比显著降低。此外，与人枸橼酸化全血接触后，接枝共聚物涂层抑制了膜上的血细胞粘附、活化和聚集。这些结果清楚地表明，聚（MPC-接枝-BMA）因其抗蛋白质吸附和抗细胞粘附性能，是改善生物医学装置血液相容性的合适材料。
