State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, People's Republic of China.
Environ Toxicol. 2013 Oct;28(10):571-8. doi: 10.1002/tox.20751. Epub 2011 Sep 2.
Using the in vivo male medaka model, we investigated the estrogen-like response of perfluorooctyl iodide (PFOI) which is a potential source of perfluorinated carboxylic acids. Using real-time quantitative polymerase chain reaction, the expression levels of related estrogenic genes including estrogenic receptor α (ERα), ERβ, vitellogenin I (VTG I), and VTG II in the livers of male medaka exposed to PFOI were analyzed. The results showed that PFOI upregulated the expression levels of the tested genes in a dose-dependent manner. VTG protein levels increased in both dose- and time-dependent manners due to PFOI exposure. The results suggested that PFOI is a potential estrogenic compound.
我们使用活体雄性青鳉鱼模型，研究了全氟辛基碘（PFOI）的类雌激素反应，它是全氟羧酸的潜在来源。通过实时定量聚合酶链反应，分析了暴露于 PFOI 的雄性青鳉鱼肝脏中相关雌激素基因，包括雌激素受体 α（ERα）、ERβ、卵黄蛋白原 I（VTG I）和卵黄蛋白原 II 的表达水平。结果表明，PFOI 以剂量依赖的方式上调了测试基因的表达水平。由于 PFOI 的暴露，VTG 蛋白水平也呈剂量和时间依赖性增加。结果表明，PFOI 是一种潜在的雌激素化合物。
