Yamaguchi Akemi, Ishibashi Hiroshi, Kohra Shinya, Arizono Koji, Tominaga Nobuaki
Department of Chemical and Biological Engineering, Ariake National College of Technology, 150 Higashihagio-machi, Omuta, Fukuoka 836-8585, Japan.
Aquat Toxicol. 2005 Apr 30;72(3):239-49. doi: 10.1016/j.aquatox.2004.12.011.
To evaluate the estrogenic activities of selected estrogenic compounds such as estradiol-17beta (E2), nonylphenol (NP), 4-(1-adamantyl)phenol (AdP), bisphenol A (BPA), BPA metabolite 4-methyl-2,4-bis(4-hydroxyphenyl)pent-1-ene (MBP) and 4,4'-dihydroxy-alpha-methylstilbene (DHMS) in the shortest possible time, we investigated the expression of estrogen-responsive genes such as vitellogenin I, vitellogenin II and alpha-type estrogen receptor genes in the liver of male medaka (Oryzias latipes) using reverse transcription-polymerase chain reaction (RT-PCR) techniques. These estrogen-responsive genes responded rapidly to selected estrogenic compounds after 8 h exposure, and the expression of hepatic vitellogenin II and estrogen receptor alpha mRNA was found to be more responsive than that of vitellogenin I mRNA. As a result, the relative estrogenic potencies of tested chemicals descended in the order of E2 (100)>MBP (0.38)>AdP (0.25)>DHMS (0.05)>NP (0.02)>BPA (0.001). Moreover, this preliminary study indicates that AdP and DHMS should be considered as candidate estrogenic compounds with the potential to induce hepatic estrogen-responsive genes in male medaka. These results suggest that vitellogenin I, vitellogenin II and estrogen receptor alpha gene expression patterns alter in male medaka treated with selected estrogenic compounds, and that these genes may be useful molecular biomarkers for screening estrogenic endocrine-disrupting chemicals in the shortest possible time.
为了在尽可能短的时间内评估选定的雌激素化合物（如17β-雌二醇（E2）、壬基酚（NP）、4-（1-金刚烷基）苯酚（AdP）、双酚A（BPA）、BPA代谢物4-甲基-2,4-双（4-羟基苯基）戊-1-烯（MBP）和4,4'-二羟基-α-甲基茋（DHMS））的雌激素活性，我们使用逆转录-聚合酶链反应（RT-PCR）技术研究了雄性青鳉（Oryzias latipes）肝脏中雌激素反应基因（如卵黄蛋白原I、卵黄蛋白原II和α型雌激素受体基因）的表达。在暴露8小时后，这些雌激素反应基因对选定的雌激素化合物迅速做出反应，并且发现肝脏卵黄蛋白原II和雌激素受体α mRNA的表达比卵黄蛋白原I mRNA更敏感。结果，受试化学物质的相对雌激素效力按E2（100）>MBP（0.38）>AdP（0.25）>DHMS（0.05）>NP（0.02）>BPA（0.001）的顺序递减。此外，这项初步研究表明，AdP和DHMS应被视为有潜力在雄性青鳉中诱导肝脏雌激素反应基因的候选雌激素化合物。这些结果表明，在用选定的雌激素化合物处理的雄性青鳉中，卵黄蛋白原I、卵黄蛋白原II和雌激素受体α基因的表达模式发生改变，并且这些基因可能是在尽可能短的时间内筛选雌激素性内分泌干扰化学物质的有用分子生物标志物。
