聚乙二胺-环糊精-替加氟缀合物具有抗癌活性和基因传递的潜力。
Polyethylenimine-cyclodextrin-tegafur conjugate shows anti-cancer activity and a potential for gene delivery.
机构信息
Institute of Chemical Biology and Pharmaceutical Chemistry, Zhejiang University, Hangzhou, China.
出版信息
J Zhejiang Univ Sci B. 2011 Sep;12(9):720-9. doi: 10.1631/jzus.B1000307.
Abstract
Polyethylenimine-cyclodextrin-tegafur (PEI-CyD-tegafur) conjugate was synthesized as a novel multifunctional prodrug of tegafur for co-delivery of chemotherapeutic agent tegafur and enhanced green fluorescent protein (EGFP) reporter plasmid DNA. Conjugation of tegafur to PEI-CyD via chemical linkage was characterized by (1)H NMR spectrometry and ultraviolet (UV) spectrometry. PEI-CyD-tegafur was able to condense plasmid DNA into complexes of around 150 nm with positive charge at the N/P ratio of 25, in accordance with electron microscopy observation of compact and monodisperse nanoparticles. The results of in vitro experiments showed enhanced cytotoxicity and considerable transfection efficiency in B16F10 cell line. Therefore, PEI-CyD-tegafur may have great potential as a co-delivery system with anti-cancer activity and potential for gene delivery.
摘要
聚乙二醇化环糊精-替加氟（PEI-CyD-tegafur）缀合物被合成作为替加氟的新型多功能前药，用于共递送化疗药物替加氟和增强型绿色荧光蛋白（EGFP）报告质粒 DNA。通过化学连接将替加氟连接到 PEI-CyD 上，通过（1）H NMR 光谱和紫外（UV）光谱进行表征。PEI-CyD-tegafur 能够将质粒 DNA 凝聚成约 150nm 的复合物，在 N/P 比为 25 时带有正电荷，这与电子显微镜观察到的紧密和单分散纳米颗粒一致。体外实验结果表明，在 B16F10 细胞系中具有增强的细胞毒性和相当高的转染效率。因此，PEI-CyD-tegafur 可能作为具有抗癌活性和基因递送潜力的共递药系统具有巨大潜力。
相似文献
1
Polyethylenimine-cyclodextrin-tegafur conjugate shows anti-cancer activity and a potential for gene delivery.聚乙二胺-环糊精-替加氟缀合物具有抗癌活性和基因传递的潜力。
J Zhejiang Univ Sci B. 2011 Sep;12(9):720-9. doi: 10.1631/jzus.B1000307.
2
[Peptide TAT modified polyethylenimine-beta-cyclodextrin for gene delivery].用于基因递送的肽TAT修饰的聚乙烯亚胺-β-环糊精
Zhejiang Da Xue Xue Bao Yi Xue Ban. 2009 Jan;38(1):15-23. doi: 10.3785/j.issn.1008-9292.2009.01.003.
3
Low molecular weight polyethylenimine cross-linked by 2-hydroxypropyl-gamma-cyclodextrin coupled to peptide targeting HER2 as a gene delivery vector.由 2-羟丙基-γ-环糊精偶联的靶向 HER2 的肽交联的低分子量聚乙二醇化亚精胺作为基因传递载体。
Biomaterials. 2010 Mar;31(7):1830-8. doi: 10.1016/j.biomaterials.2009.11.012. Epub 2009 Nov 25.
4
[Peptide CY11 conjugated polyethylenimine-beta-cyclodextrin for gene delivery].用于基因递送的肽CY11偶联聚乙烯亚胺-β-环糊精
Zhejiang Da Xue Xue Bao Yi Xue Ban. 2009 Jan;38(1):24-30. doi: 10.3785/j.issn.1008-9292.2009.01.004.
5
FGF receptor-mediated gene delivery using ligands coupled to PEI-β-CyD.使用与聚乙二醇化β-环糊精偶联的配体进行成纤维细胞生长因子受体介导的基因递送。
J Biomed Biotechnol. 2012;2012:989235. doi: 10.1155/2012/989235. Epub 2012 Apr 11.
6
[Peptide MC10 mediated PEI-beta-CyD as a gene delivery vector targeting to Her-2 receptor].[肽MC10介导的聚乙烯亚胺-β-环糊精作为靶向人表皮生长因子受体2的基因递送载体]
Zhejiang Da Xue Xue Bao Yi Xue Ban. 2009 Jan;38(1):7-14. doi: 10.3785/j.issn.1008-9292.2009.01.002.
7
Chitosan-graft-(PEI-β-cyclodextrin) copolymers and their supramolecular PEGylation for DNA and siRNA delivery.壳聚糖接枝-(PEI-β-环糊精)共聚物及其超分子聚乙二醇化用于 DNA 和 siRNA 的递送。
Biomaterials. 2011 Nov;32(32):8328-41. doi: 10.1016/j.biomaterials.2011.07.038. Epub 2011 Aug 15.
8
MC8 peptide-mediated Her-2 receptor targeting based on PEI-β-CyD as gene delivery vector.基于 PEI-β-CyD 的 MC8 肽介导的 Her-2 受体靶向作为基因传递载体。
Appl Biochem Biotechnol. 2013 Jan;169(2):450-61. doi: 10.1007/s12010-012-9959-2. Epub 2012 Dec 8.
9
Cationic star polymers consisting of alpha-cyclodextrin core and oligoethylenimine arms as nonviral gene delivery vectors.由α-环糊精核心和低聚乙二胺臂组成的阳离子星形聚合物作为非病毒基因传递载体。
Biomaterials. 2007 Jul;28(21):3245-54. doi: 10.1016/j.biomaterials.2007.03.033. Epub 2007 Apr 12.
10
FGF receptor-mediated gene delivery using ligands coupled to polyethylenimine.使用与聚乙烯亚胺偶联的配体进行成纤维细胞生长因子受体介导的基因递送。
J Biomater Appl. 2007 Sep;22(2):163-80. doi: 10.1177/0885328206074503. Epub 2007 Jan 25.
引用本文的文献
1
New MoS/Tegafur-Containing Pharmaceutical Formulations for Selective LED-Based Skin Cancer Photo-Chemotherapy.用于基于LED的选择性皮肤癌光化学疗法的含新型丝裂霉素/替加氟药物制剂
Pharmaceutics. 2024 Mar 4;16(3):360. doi: 10.3390/pharmaceutics16030360.
2
Cyclodextrins in non-viral gene delivery.环糊精在非病毒基因传递中的应用。
Biomaterials. 2014 Jan;35(1):401-11. doi: 10.1016/j.biomaterials.2013.09.061. Epub 2013 Oct 5.
3
Low-weight polyethylenimine cross-linked 2-hydroxypopyl-β-cyclodextrin and folic acid as an efficient and nontoxic siRNA carrier for gene silencing and tumor inhibition by VEGF siRNA.低分子量聚亚乙基亚胺交联 2-羟丙基-β-环糊精和叶酸作为高效无毒的 siRNA 载体，通过 VEGF siRNA 抑制基因表达和肿瘤生长。
Int J Nanomedicine. 2013;8:2101-17. doi: 10.2147/IJN.S42440. Epub 2013 Jun 5.
本文引用的文献
1
Low molecular weight polyethylenimine cross-linked by 2-hydroxypropyl-gamma-cyclodextrin coupled to peptide targeting HER2 as a gene delivery vector.由 2-羟丙基-γ-环糊精偶联的靶向 HER2 的肽交联的低分子量聚乙二醇化亚精胺作为基因传递载体。
Biomaterials. 2010 Mar;31(7):1830-8. doi: 10.1016/j.biomaterials.2009.11.012. Epub 2009 Nov 25.
2
Co-delivery of doxorubicin and Bcl-2 siRNA by mesoporous silica nanoparticles enhances the efficacy of chemotherapy in multidrug-resistant cancer cells.介孔二氧化硅纳米颗粒共递送阿霉素和Bcl-2小干扰RNA可增强多药耐药癌细胞的化疗效果。
Small. 2009 Dec;5(23):2673-7. doi: 10.1002/smll.200900621.
3
Preparation and characterization of tegafur magnetic thermosensitive liposomes.替加氟磁性热敏脂质体的制备与表征
Pharm Dev Technol. 2009;14(4):350-7. doi: 10.1080/10837450802647300.
4
The gene transfection efficiency of a folate-PEI600-cyclodextrin nanopolymer.叶酸-PEI600-环糊精纳米聚合物的基因转染效率
Biomaterials. 2009 Oct;30(29):5793-803. doi: 10.1016/j.biomaterials.2009.06.051. Epub 2009 Jul 16.
5
[Preparation of floxuridine loaded polycation and its antitumor activity].[载氟尿苷聚阳离子的制备及其抗肿瘤活性]
Zhejiang Da Xue Xue Bao Yi Xue Ban. 2009 Jan;38(1):53-8. doi: 10.3785/j.issn.1008-9292.2009.01.008.
6
Nonviral vectors for gene delivery.用于基因递送的非病毒载体。
Chem Rev. 2009 Feb;109(2):259-302. doi: 10.1021/cr800409e.
7
Study of carbonyl iron/poly(butylcyanoacrylate) (core/shell) particles as anticancer drug delivery systems Loading and release properties.羰基铁/聚（氰基丙烯酸丁酯）（核/壳）颗粒作为抗癌药物递送系统的研究 负载和释放特性。
Eur J Pharm Sci. 2008 Mar 3;33(3):252-61. doi: 10.1016/j.ejps.2007.12.005. Epub 2007 Dec 23.
8
Novel prodrugs of tegafur that display improved anticancer activity and antiangiogenic properties.具有改善的抗癌活性和抗血管生成特性的替加氟新型前药。
J Med Chem. 2008 Jan 24;51(2):314-23. doi: 10.1021/jm7009827. Epub 2007 Dec 29.
9
Tegafur loading and release properties of magnetite/poly(alkylcyanoacrylate) (core/shell) nanoparticles.替加氟在磁铁矿/聚（烷基氰基丙烯酸酯）（核/壳）纳米颗粒中的负载和释放特性。
J Control Release. 2008 Jan 4;125(1):50-8. doi: 10.1016/j.jconrel.2007.09.008. Epub 2007 Sep 26.
10
Safety evaluation of oral fluoropyrimidine S-1 for short- and long-term delivery in advanced gastric cancer: analysis of 3,758 patients.口服氟嘧啶S-1用于晚期胃癌短期和长期给药的安全性评估：3758例患者的分析
Cancer Chemother Pharmacol. 2008 Feb;61(2):335-43. doi: 10.1007/s00280-007-0595-4. Epub 2007 Oct 9.
Zotero 插件