一种商业逆转录聚合酶链反应（RT-PCR）试剂中存在鼠白血病病毒（MLV）相关病毒基因序列。
Presence of murine leukemia virus (MLV)-related virus gene sequences in a commercial RT-PCR reagent.
作者信息
Wolff Dietmar, Gerritzen Andreas
机构信息
Medical Laboratory Bremen, Bremen, Germany.
出版信息
Clin Lab. 2011;57(7-8):631-4.
BACKGROUND
The recent identification of murine leukemia virus (MLV)-related viruses in patients with chronic fatigue syndrome (CFS) has aroused much interest, not least among sufferers. However, other studies failed to detect these viruses in CFS patients.
METHODS
We wanted to establish a MLV-related virus real-time PCR for routine diagnostics.
RESULTS
Our study identified false positive MLV-related virus results due to a contamination of Superscript III Platinum One-Step Quantitative RT-PCR System (Invitrogen).
CONCLUSIONS
This observation may be helpful to elucidate discrepant results for the detection of MLV-related virus like xenotropic MLV-related virus (XMRV) in recently published studies.
背景
近期在慢性疲劳综合征（CFS）患者中发现与鼠白血病病毒（MLV）相关的病毒，这引起了广泛关注，尤其是在患者群体中。然而，其他研究未能在CFS患者中检测到这些病毒。
方法
我们希望建立一种用于常规诊断的与MLV相关病毒的实时PCR方法。
结果
我们的研究发现，由于SuperScript III Platinum一步定量逆转录PCR系统（Invitrogen公司）受到污染，导致与MLV相关病毒的结果出现假阳性。
结论
这一观察结果可能有助于解释近期发表的研究中在检测与MLV相关病毒（如嗜异性MLV相关病毒，XMRV）时出现的不一致结果。
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