de Barros João Bosco Gonçalves, de Paula Tarcízio Antônio Rego, da Matta Sérgio Luis Pinto, Fonseca Cláudio César, Leite Flaviana Lima Guião, Rossi João Luiz, de Oliveira Priscila Carvalho, da Costa Eduardo Paulino
Veterinary Department, Federal University of Viçosa, Minas Gerais 36571-000, Brazil.
Anim Reprod Sci. 2007 Dec;102(3-4):350-6. doi: 10.1016/j.anireprosci.2007.04.002. Epub 2007 Apr 8.
The intrinsic yield of spermatogenesis and the supporting indexes of the Sertoli cells are the best indicators for the spermatic production capacity in a species. The aim of the present study was to quantify the intrinsic yield of the spermatogenetic process, as well as the Sertoli cell index and spermatic reserves. Testicular fragments of five adult African lions was fixed in 4% glutaric aldehyde, dehydrated at increasing alcohol concentrations, included into hydroxyethyl methacrylate, and were cut into 4 microm thickness. In the seminiferous epithelium of the African lions, 10.3 primary spermatocytes at pre-leptotene phase are produced by the type-A spermatogonia. During meiotic divisions, only 2.7 spermatids were produced from the primary spermatocytes. The general spermatogenesis production in the African lions was approximately 22.1 cells, and each Sertoli cell was able to sustain and maintain approximately 14.9 cells of the germinative line, from which 7.9 are round spermatids. A total of 103x10(6) spermatozoa are produced by each testis gram at each cycle of the seminiferous epithelium. The spermatic reserve of lion is below the amplitude observed in mammals.
精子发生的内在产量以及支持细胞的相关指标是一个物种精子产生能力的最佳指标。本研究的目的是量化精子发生过程的内在产量，以及支持细胞指数和精子储备。将五只成年非洲狮的睾丸组织块固定于4%戊二醛中，依次用浓度递增的酒精脱水，包埋于甲基丙烯酸羟乙酯中，切成4微米厚的切片。在非洲狮的生精上皮中，A型精原细胞产生10.3个前细线期初级精母细胞。在减数分裂过程中，初级精母细胞仅产生2.7个精子细胞。非洲狮的总体精子发生产量约为22.1个细胞，每个支持细胞能够维持约14.9个生殖系细胞，其中7.9个是圆形精子细胞。在生精上皮的每个周期中，每克睾丸组织每个睾丸产生总计103×10⁶个精子。狮子的精子储备低于在哺乳动物中观察到的幅度。
