Laboratory of Molecular Brain Science, Graduate School of Integrated Arts and Sciences, Hiroshima University, Higashi-Hiroshima 739-8521, Japan.
J Biosci Bioeng. 2011 Dec;112(6):638-42. doi: 10.1016/j.jbiosc.2011.08.005. Epub 2011 Sep 1.
The anti-oxidative activity of the rare sugar D-allose has recently been reported, but the mechanism is largely unclear. In this study, we evaluated the reactive oxygen species (ROS) scavenging activities of D-allose and then examined the effects of D-allose on ROS production in mitochondria to clarify the antioxidant properties of D-allose. While D-allose did not scavenge hydrogen peroxide and superoxide anions, it eliminated hydroxyl radicals to the same extent as D-glucose. Rotenone, an uncoupler of mitochondrial respiratory complex I, induces ROS production in mouse neuroblastoma Neuro2A cells in the presence of D-glucose. However, in the presence of D-allose, there was no change in the ROS levels in Neuro2A cells following rotenone treatment. Furthermore, treatment with D-allose attenuated the D-glucose-dependent ROS generation induced by rotenone. Whereas treatment with D-glucose enhanced ATP synthesis in Neuro2A cells, D-allose was less effective in producing intracellular ATP than D-glucose. Treatment with D-allose inhibited the ATP synthesis stimulated by D-glucose. These results suggest that D-allose suppresses ROS production in the mitochondria due to competition with D-glucose at the cellular level.
D-艾杜糖的抗氧化活性最近已经有报道，但其机制尚不清楚。在这项研究中，我们评估了 D-艾杜糖对活性氧（ROS）的清除活性，然后研究了 D-艾杜糖对线粒体中 ROS 产生的影响，以阐明 D-艾杜糖的抗氧化特性。虽然 D-艾杜糖不能清除过氧化氢和超氧阴离子，但它对羟自由基的清除能力与 D-葡萄糖相同。在 D-葡萄糖存在的情况下，鱼藤酮（一种线粒体呼吸复合物 I 的解偶联剂）会诱导小鼠神经母细胞瘤 Neuro2A 细胞中 ROS 的产生。然而，在 D-艾杜糖存在的情况下，鱼藤酮处理后 Neuro2A 细胞中的 ROS 水平没有变化。此外，D-艾杜糖处理可减轻鱼藤酮诱导的 D-葡萄糖依赖性 ROS 生成。虽然 D-葡萄糖处理增强了 Neuro2A 细胞中的 ATP 合成，但 D-艾杜糖在产生细胞内 ATP 方面不如 D-葡萄糖有效。D-艾杜糖处理抑制了 D-葡萄糖刺激的 ATP 合成。这些结果表明，D-艾杜糖通过在细胞水平上与 D-葡萄糖竞争，抑制线粒体中 ROS 的产生。
