Department of Internal Medicine, Division of Infectious Diseases, University Medical Center Groningen, The Netherlands.
Clin Immunol. 2011 Nov;141(2):161-8. doi: 10.1016/j.clim.2011.07.004. Epub 2011 Jul 30.
Influenza-specific cell-mediated immune (CMI) responses can protect from influenza, but may be decreased in CVID-patients since defects in CMI responses have been demonstrated in CVID-patients. Therefore CMI responses were evaluated in 15 CVID-patients and 15 matched healthy controls (HC) by determining frequencies of interferon (IFN)γ-producing PBMC, and frequencies of IFNγ-, interleukin (IL)-2- and tumour necrosis factor (TNF)α-producing CD4+ and CD8+ T-cells before and after influenza vaccination using IFNγ enzyme-linked immunospot (IFNγ-ELISpot) and flow cytometry. Humoral responses were determined using haemagglutination inhibition assay. In CVID-patients the number of spotforming PBMC in the IFNγ-ELISpot did not increase following influenza vaccination, in contrast to HC. In flow cytometry, the frequencies of IFNγ-producing T-cells decreased in CVID-patients after influenza vaccination, while in HC the frequencies of IFNγ-production flow cytometry increased. Concluding, CMI responses following influenza vaccination are hampered in CVID-patients compared to HC. Additional protective strategies against influenza other than vaccination are warranted.
流感特异性细胞介导免疫（CMI）反应可提供针对流感的保护，但由于 CVID 患者的 CMI 反应存在缺陷，因此 CVID 患者的 CMI 反应可能会降低。因此，通过使用 IFNγ酶联免疫斑点（IFNγ-ELISpot）和流式细胞术，在流感疫苗接种前后，测定干扰素（IFN）γ产生的 PBMC 频率以及 IFNγ-、白细胞介素（IL）-2-和肿瘤坏死因子（TNF）α产生的 CD4+和 CD8+ T 细胞的频率，评估了 15 名 CVID 患者和 15 名匹配的健康对照（HC）的 CMI 反应。使用血凝抑制测定法确定了体液反应。在 CVID 患者中，与 HC 相比，流感疫苗接种后 IFNγ-ELISpot 中形成斑点的 PBMC 数量并未增加。在流式细胞术分析中，CVID 患者流感疫苗接种后 IFNγ 产生 T 细胞的频率下降，而 HC 中 IFNγ 产生的流式细胞术增加。综上所述，与 HC 相比，CVID 患者接种流感疫苗后的 CMI 反应受到阻碍。需要针对流感的其他额外保护策略，而不仅仅是接种疫苗。
