Department of Biotechnology and Microbiology, Kannur University, Thalassery Campus, 670661 Kerala, India.
Int J Biol Macromol. 2011 Dec 1;49(5):992-8. doi: 10.1016/j.ijbiomac.2011.08.021. Epub 2011 Aug 26.
A galactose-specific seed lectin from Spatholobous parviflorus (SPL) has been purified, crystallized and its X-ray structure solved. It is the first lectin purified and crystallized from the genus Spatholobus (family: Fabaceae). The crystals belong to the space group P1, with a=60.792 Å, b=60.998 Å, c=78.179 Å, α=78.68°, β=88.62°, γ=104.32°. The data were collected at 2.04 Å resolution under cryocondition, on a MAR image-plate detector system, mounted on a rotating anode X-ray generator. The coordinates of Dolichos biflorus lectin (1lu1) were successfully used for the structure solution by molecular replacement method. The primary structure of the SPL was not known earlier and it was unambiguously visible in the electron density. S. parviflorus lectin is a hetero-dimeric-tetramer with two alpha and two beta chains of 251 and 239 residues respectively. SPL has two metal ions, Ca(2+) and Mn(2+), bound to a loop region of each chain. The SPL monomers are in jelly roll form.
已从美丽崖豆藤（Spatholobous parviflorus）中纯化、结晶并解析出其 X 射线结构的半乳糖特异性种籽凝集素。这是从崖豆藤属（豆科）中纯化和结晶出的第一种凝集素。晶体属于 P1 空间群，具有 a=60.792 Å、b=60.998 Å、c=78.179 Å、α=78.68°、β=88.62°、γ=104.32°。在 cryocondition 下，使用 MAR 图像板探测器系统在旋转阳极 X 射线发生器上以 2.04 Å 的分辨率收集数据。成功使用多花豇豆凝集素（1lu1）的坐标通过分子置换法解决结构。SPL 的一级结构以前未知，在电子密度中清晰可见。美丽崖豆藤凝集素是一种异源二聚体-四聚体，分别由 251 和 239 个残基的两条α和两条β链组成。SPL 结合有两个金属离子 Ca（2+）和 Mn（2+），分别结合在每条链的环区。SPL 单体呈果冻卷形式。
