Innovative Seed Lab (ISL), Faculty of Biomedical and Health Sciences, Universidad Europea de Madrid, 28670 Villaviciosa de Odón, Spain.
Int J Mol Sci. 2024 Sep 3;25(17):9553. doi: 10.3390/ijms25179553.
Fungi infection, especially derived from , causes severe grapevine economic losses worldwide. Despite the availability of chemical treatments, looking for eco-friendly ways to control infection is gaining much more attention. When a plant is infected, multiple disease-control molecular mechanisms are activated. PRRs (Pattern Recognition Receptors) and particularly RLKs (receptor-like kinases) take part in the first barrier of the immune system, and, as a consequence, the kinase signaling cascade is activated, resulting in an immune response. In this context, discovering new lectin-RLK (LecRLK) membrane-bounded proteins has emerged as a promising strategy. The genome-wide localization of potential LecRLKs involved in disease defense was reported in two grapevine varieties of great economic impact: Chardonnay and Pinot Noir. A total of 23 potential amino acid sequences were identified, exhibiting high-sequence homology and evolution related to tandem events. Based on the domain architecture, a carbohydrate specificity ligand assay was conducted with docking, revealing two sequences as candidates for specific host-pathogen interaction. This study confers a starting point for designing new effective antifungal treatments directed at LecRLK targets in .
真菌感染，特别是由 引起的感染，在全球范围内给葡萄种植业造成了严重的经济损失。尽管有化学处理方法，但寻找环保的方法来控制 感染越来越受到关注。当植物受到感染时，多种疾病控制的分子机制被激活。PRRs（模式识别受体）和特别是 RLKs（受体样激酶）参与免疫系统的第一道防线，因此，激酶信号级联被激活，导致免疫反应。在这种情况下，发现新的凝集素-RLK（LecRLK）膜结合蛋白已成为一种有前途的策略。在两个具有重大经济影响的葡萄品种（霞多丽和黑比诺）中，报告了参与疾病防御的潜在 LecRLKs 的全基因组定位。总共鉴定了 23 个潜在的氨基酸序列，它们具有高度的序列同源性和与串联事件相关的进化。基于结构域架构，进行了带有对接的碳水化合物特异性配体测定，揭示了两个序列作为特定宿主-病原体相互作用的候选物。本研究为设计针对 中 LecRLK 靶标的新型有效抗真菌治疗方法提供了起点。
