Kieft T L, Ruscetti T
Department of Biology, New Mexico Institute of Mining and Technology, Socorro 87801.
J Bacteriol. 1990 Jun;172(6):3519-23. doi: 10.1128/jb.172.6.3519-3523.1990.
Biological ice nuclei (active at approximately -4 degrees C) were extracted from cells of the lichen Rhizoplaca chrysoleuca by sonication. Sensitivity to proteases, guanidine hydrochloride, and urea showed these nuclei to be proteinaceous. The nuclei were relatively heat stable, active from pH 1.5 to 12, and active without lipids, thereby demonstrating significant differences from bacterial ice nuclei.
通过超声处理从地衣黄髓根瘤菌的细胞中提取了生物冰核（在约-4℃时具有活性）。对蛋白酶、盐酸胍和尿素的敏感性表明这些冰核是蛋白质性质的。这些冰核相对耐热，在pH值1.5至12范围内具有活性，且在没有脂质的情况下也具有活性，从而显示出与细菌冰核的显著差异。
