Department of Pharmacology and Clinical Neuroscience, Umeå University, SE-90187 Umeå, Sweden.
Toxicol Lett. 2011 Nov 10;207(1):1-6. doi: 10.1016/j.toxlet.2011.08.014. Epub 2011 Aug 26.
It is now well established that nicotine adversely affects the integrity of the blood-brain barrier (BBB). In contrast, nicotine has been reported to increase the transendothelial electrical resistance (TEER) of CaCo₂ colon cancer cells. In the present study, the effects of nicotine upon the TEER and sucrose permeability of ECV304/C6 co-cultures and, for comparative purposes, CaCo₂ cells has been investigated. Neither ECV304 nor C6 cells were found to express measurable membrane levels of nicotinic acetylcholine receptors, as assessed by [³H]-epibatidine binding. Nicotine treatment (0.01-1 μM) for up to 48 h had little or no effect upon the TEER or sucrose permeability of either ECV304/C6 co-cultures or CaCo₂ cells. It is concluded that in contrast to the situation for the BBB, ECV304 cells lack nicotinic acetylcholine receptors and the barrier properties of ECV304/C6 co-cultures are not affected to any important extent by nicotine. This study underlines the conclusions made by other authors that the ECV304/C6 co-culture system is of limited validity as a model of the BBB.
现在已经证实，尼古丁会对血脑屏障（BBB）的完整性产生不利影响。相比之下，有报道称尼古丁会增加 CaCo₂ 结肠癌细胞的跨内皮电阻（TEER）。在本研究中，研究了尼古丁对 ECV304/C6 共培养物的 TEER 和蔗糖通透性的影响，并为了进行比较，还研究了 CaCo₂ 细胞。通过 [³H]-epibatidine 结合评估，发现无论是 ECV304 还是 C6 细胞都没有表达可测量的烟碱型乙酰胆碱受体膜水平。尼古丁处理（0.01-1 μM）长达 48 小时，对 ECV304/C6 共培养物或 CaCo₂ 细胞的 TEER 或蔗糖通透性几乎没有影响。因此可以得出结论，与 BBB 的情况相反，ECV304 细胞缺乏烟碱型乙酰胆碱受体，ECV304/C6 共培养物的屏障特性不会受到尼古丁的重要影响。这项研究强调了其他作者的结论，即 ECV304/C6 共培养系统作为 BBB 模型的有效性有限。
