Iizawa Y, Nakao M, Kondo M, Yamazaki T
Research and Development Division, Takeda Chemical Industries, Ltd., Osaka.
Microbiol Immunol. 1990;34(2):185-95. doi: 10.1111/j.1348-0421.1990.tb01003.x.
The effects of recombinant human interleukin-2 (rIL-2) administered prophylactically on the death of CBA/J mice challenged with Klebsiella pneumoniae 27 intraperitoneally were examined. rIL-2 administered subcutaneously at 20 micrograms per mouse for 7 days enhanced survival after a lethal challenge. The injection of anti-asialo GM1 antibody did not influence the effect of rIL-2. In mice given rIL-2, the number of peritoneal macrophages increased, and the infiltration of polymorphonuclear leukocytes (PMN) into the peritoneal cavity after the bacterial challenge was enhanced. In addition, adoptive transfer of sera and peritoneal exudate cells (PEC), consisting of an approximately equal number of macrophages and PMN, obtained from mice given rIL-2 enhanced resistance to a K. pneumoniae infection, compared with adoptive transfer of sera and PEC obtained from mice not given rIL-2. These results indicate that rIL-2 protects mice from a lethal challenge with K. pneumoniae, and suggest that the protective effect is due to an increase in the number of phagocytic cells and in the cooperative activity of the sera and the phagocytic cells.
研究了预防性给予重组人白细胞介素-2（rIL-2）对腹腔注射肺炎克雷伯菌27株攻击的CBA/J小鼠死亡的影响。以每只小鼠20微克的剂量皮下注射rIL-2，连续7天，可提高致死性攻击后的存活率。注射抗去唾液酸GM1抗体不影响rIL-2的作用。给予rIL-2的小鼠腹腔巨噬细胞数量增加，细菌攻击后多形核白细胞（PMN）向腹腔的浸润增强。此外，与未给予rIL-2的小鼠的血清和腹腔渗出细胞（PEC）的过继转移相比，给予rIL-2的小鼠的血清和PEC（由数量大致相等的巨噬细胞和PMN组成）的过继转移增强了对肺炎克雷伯菌感染的抵抗力。这些结果表明，rIL-2可保护小鼠免受肺炎克雷伯菌的致死性攻击，并提示保护作用归因于吞噬细胞数量的增加以及血清与吞噬细胞的协同活性。
