Kawakita T, Yamada A, Mitsuyama M, Kumazawa Y, Nomoto K
Department of Immunology, Kyushu University, Fukuoka, Japan.
Immunopharmacol Immunotoxicol. 1987;9(4):523-40. doi: 10.3109/08923978709035230.
Survival of mice after intraperitoneal (ip) or intravenous (iv) infection with Pseudomonas aeruginosa was augmented in the mice that had been pretreated ip with a Chinese traditional herbal medicine, xiao-chai-hu-tang (Japanese name: shosaiko-to) 6 hours or 4 days previously. 1) The pretreatment with shosaiko-to 6 hours previously induced an accumulation of polymorphonuclear leukocytes (PMN) in the peritoneal cavity, and its protective effect against ip infection was not impaired by treatment with carrageenan, a macrophage blocking agent. These results suggested that the protective effects of shosaiko-to against P. aeruginosa infection depended mainly on PMN in mice pretreated at this timing. 2) The pretreatment with shosaiko-to 4 days previously induced an accumulation of macrophages showing an augmented phagocytosis of P. aeruginosa in vitro in the presence of immune serum, and its protective effect against P. aeruginosa was impaired by treatment with carrageenan. In addition, the pretreatment with shosaiko-to accelerated the bacterial clearance from the blood. The sera obtained from mice treated with shosaiko-to 4 days previously showed a high titer of antibody specific to P. aeruginosa. When this sera was transferred to naive mice, these recipients showed an accelerated bacterial clearance and an increased survival to challenge infection with P. aeruginosa. These results suggested that protective effects of shosaiko-to against P. aeruginosa infection at this timing depended on cooperation of macrophages and antibody which produced by stimulation of shosaiko-to, a polyclonal B cell activator. Such polyclonal antibodies were also effective on protection against encapsulated Klebsiella pneumoneae to which antibody was essential in the expression of resistance. These results suggested that shosaiko-to could augment nonspecific resistance to a variety of bacteria to which antibody plays an effective role.
在腹腔内（ip）或静脉内（iv）感染铜绿假单胞菌后，预先在6小时或4天前经腹腔注射一种中药小柴胡汤（日本名：柴胡汤）进行预处理的小鼠存活率提高。1）6小时前用柴胡汤预处理可诱导多形核白细胞（PMN）在腹腔内积聚，其对腹腔感染的保护作用不受巨噬细胞阻断剂角叉菜胶处理的影响。这些结果表明，在此时间点预处理的小鼠中，柴胡汤对铜绿假单胞菌感染的保护作用主要取决于PMN。2）4天前用柴胡汤预处理可诱导巨噬细胞积聚，在免疫血清存在下，巨噬细胞在体外对铜绿假单胞菌的吞噬作用增强，其对铜绿假单胞菌的保护作用因角叉菜胶处理而受损。此外，柴胡汤预处理加速了血液中细菌的清除。4天前用柴胡汤处理的小鼠血清显示出高滴度的铜绿假单胞菌特异性抗体。当将这种血清转移到未感染的小鼠时，这些受体显示出细菌清除加速和对铜绿假单胞菌攻击感染的存活率增加。这些结果表明，此时柴胡汤对铜绿假单胞菌感染的保护作用取决于巨噬细胞和由柴胡汤刺激产生的抗体的协同作用，柴胡汤是一种多克隆B细胞激活剂。这种多克隆抗体对预防包膜肺炎克雷伯菌感染也有效，抗体对其抗性表达至关重要。这些结果表明，柴胡汤可以增强对多种细菌的非特异性抗性，抗体在其中发挥有效作用。
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