Shirsat N V, Pignolo R J, Kreider B L, Rovera G
Wistar Institute, Philadelphia, Pennsylvania 19104.
Oncogene. 1990 May;5(5):769-72.
A 1.26 kb murine cDNA having 31% homology with human ras and 55% homology with human rho proteins was isolated using an oligonucleotide probe homologous to catalytic subdomain of a tyrosine kinase subfamily. Northern blot analysis indicates that the expression of the murine gene is restricted to the cells of hemopoietic lineages and the mRNA levels increase with the terminal differentiation of hemopoietic cells into granulocytes.
利用与酪氨酸激酶亚家族催化亚结构域同源的寡核苷酸探针，分离出了一个1.26kb的小鼠cDNA，它与人类ras蛋白有31%的同源性，与人类rho蛋白有55%的同源性。Northern印迹分析表明，该小鼠基因的表达仅限于造血谱系的细胞，并且随着造血细胞终末分化为粒细胞，mRNA水平升高。
