Department of Gynaecology and Obstetrics, Fourth Hospital of Harbin Medical University, Harbin, China.
Int J Gynecol Cancer. 2011 Oct;21(7):1166-71. doi: 10.1097/IGC.0b013e31822c76e1.
To investigate the expression of the nucleostemin (NS) gene in ovarian tumors and its correlation with the expression of cyclin D1.
The expression levels of nucleostemin and cyclin D1 proteins were measured by immunohistochemical staining in ovarian tumors and normal ovarian tissues, and the relationship between their levels was analyzed.
Nucleostemin gene and cyclin D1 expressions were detected in 28 and 27 specimens of malignant ovarian tumors (93.7% and 90.0%), 4 and 8 specimens of ovarian borderline tumors (40.0% and 80.0%), and in both specimens of benign ovarian tumors, respectively. The expression of NS gene was seen in 5, 13, and 10 specimens of malignant ovarian tumors of high, moderate, and low grade, respectively. The expression of the nucleostemin gene was significantly related to the tumor grade (r = 0.786, P < 0. 05), and a significant relationship between nucleostemin and cyclin D1 expression was found (r = 0.834, P < 0.05). The expression of the nucleostemin gene was not detected in normal ovarian tissue.
The expression of the nucleostemin gene in ovarian tumors is closely correlated with origination, progression, and grading of tumors and may serve as a marker in estimating the malignancy of ovarian tumors. The overexpression of cyclin D1 might be correlated with nucleostemin expression. Nucleostemin may have an impact on the passage of cells through the G1/S checkpoint, and thus cell cycle progress, by regulating cyclin D1 expression.
研究核干细胞（NS）基因在卵巢肿瘤中的表达及其与细胞周期蛋白 D1 的表达的相关性。
采用免疫组化法检测卵巢肿瘤及正常卵巢组织中核干细胞和细胞周期蛋白 D1 蛋白的表达水平，并分析其水平的相关性。
28 例恶性卵巢肿瘤（93.7%和 90.0%）、4 例卵巢交界性肿瘤（40.0%和 80.0%）和 2 例良性卵巢肿瘤中检测到核干细胞基因和细胞周期蛋白 D1 的表达。在恶性卵巢肿瘤中，高、中、低分化肿瘤中核干细胞基因的表达分别为 5、13、10 例。核干细胞基因的表达与肿瘤分级显著相关（r = 0.786，P < 0.05），并发现核干细胞与细胞周期蛋白 D1 表达之间存在显著相关性（r = 0.834，P < 0.05）。正常卵巢组织中未检测到核干细胞基因的表达。
卵巢肿瘤中核干细胞基因的表达与肿瘤的起源、进展和分级密切相关，可作为评估卵巢肿瘤恶性程度的标志物。细胞周期蛋白 D1 的过表达可能与核干细胞的表达有关。核干细胞可能通过调节细胞周期蛋白 D1 的表达影响细胞通过 G1/S 检验点的传递，从而影响细胞周期的进展。
