Wang Jiao, Wang Lu, Ji Qingchun, Zhu Hong, Han Shiyu
Department of Gynecology and Obstetrics, The Fourth Hospital of Harbin Medical University, Harbin, 150001, China.
Department of Gynecology and Obstetrics, The Fourth Hospital of Harbin Medical University, Harbin, 150001, China.
Biochem Biophys Res Commun. 2017 May 27;487(2):262-267. doi: 10.1016/j.bbrc.2017.04.046. Epub 2017 Apr 12.
Nucleostemin plays an essential role in the proliferation of some stem cells and cancer cells, but no research has been conducted to assess the link between Nucleostemin and ovarian cancer. To investigate the role of Nucleostemin in ovarian cancer tumorigenesis, we generated short hairpin (sh)RNA to knockdown the expression of the Nucleostemin gene in an ovarian cancer SKOV-3 cell line. We found that knockdown of this gene led to cell-cycle arrest, as well as to an increase in apoptosis. In addition, migration and invasion demonstrated significant inhibition in Nucleostemin-deficient cells. Furthermore, the knockdown of Nucleostemin dramatically suppressed xenograft progression in BALB/c nude mice. Our findings suggest that Nucleostemin is associated with malignancy in an ovarian cancer SKOV-3 cell line.
核仁素在某些干细胞和癌细胞的增殖中起着至关重要的作用，但尚未开展研究来评估核仁素与卵巢癌之间的联系。为了研究核仁素在卵巢癌发生中的作用，我们构建了短发夹（sh）RNA以敲低卵巢癌SKOV-3细胞系中核仁素基因的表达。我们发现该基因的敲低导致细胞周期停滞以及细胞凋亡增加。此外，在缺乏核仁素的细胞中，迁移和侵袭表现出显著抑制。此外，核仁素的敲低显著抑制了BALB/c裸鼠体内异种移植瘤的进展。我们的研究结果表明，核仁素与卵巢癌SKOV-3细胞系的恶性程度相关。
