迈向人体血液源性 miRNA 组学与人类疾病
Toward the blood-borne miRNome of human diseases.
机构信息
Biomarker Discovery Center, Heidelberg, Germany.
出版信息
Nat Methods. 2011 Sep 4;8(10):841-3. doi: 10.1038/nmeth.1682.
In a multicenter study, we determined the expression profiles of 863 microRNAs by array analysis of 454 blood samples from human individuals with different cancers or noncancer diseases, and validated this 'miRNome' by quantitative real-time PCR. We detected consistently deregulated profiles for all tested diseases; pathway analysis confirmed disease association of the respective microRNAs. We observed significant correlations (P = 0.004) between the genomic location of disease-associated genetic variants and deregulated microRNAs.
在一项多中心研究中，我们通过对 454 份来自患有不同癌症或非癌症疾病的个体的血液样本进行微阵列分析，确定了 863 个 microRNAs 的表达谱，并通过定量实时 PCR 对该“miRNome”进行了验证。我们检测到所有测试疾病的 microRNA 表达谱均存在一致性下调；通路分析证实了相应 microRNAs 与疾病的关联。我们观察到疾病相关遗传变异的基因组位置与下调的 microRNAs 之间存在显著相关性（P=0.004）。
Zotero 插件