Laboratory of Proteins and Carbohydrates of Marine Algae, Department of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza, 60455-760, CE, Brazil.
Laboratory of Proteins and Carbohydrates of Marine Algae, Department of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza, 60455-760, CE, Brazil; University Center Inta (UNINTA), Campus of Itapipoca, 62501-040, CE, Brazil.
Int J Biol Macromol. 2020 Sep 15;159:415-421. doi: 10.1016/j.ijbiomac.2020.05.012. Epub 2020 May 6.
A sulfated polysaccharide from the red algae Gelidiella acerosa (GaSP) was obtained through enzymatic extraction and subjected to chemical characterization by HPSEC, elemental microanalysis, FT-IR and NMR spectroscopies. The GaSP anticoagulant activity was investigated through APTT and PT tests and platelet aggregation assessed by turbidimetry. The antithrombotic and hemorrhagic activities were evaluated by venous thrombosis and hemorrhagic tendency models, respectively. FT-IR and NMR demonstrated that GaSP is a sulfated agaran. HPSEC and elemental microanalysis revealed a peak molar mass of 284.8 kDa and a degree of sulfation of 0.63, respectively. This molecule prolonged the coagulation time in 2.1 times and inhibited the platelet aggregation by 45%. Furthermore, it showed significant dose-dependent antithrombotic effect of 40%, 64% and 80% at 0.1, 0.5 and 1 mg/kg, respectively, without hemorrhage. These results suggest that GaSP has promising antithrombotic.
从红藻石花菜（GaSP）中获得了一种硫酸化多糖，通过酶提取并通过 HPSEC、元素微量分析、FT-IR 和 NMR 光谱进行化学表征。通过 APTT 和 PT 试验研究了 GaSP 的抗凝活性，并通过浊度法评估血小板聚集。通过静脉血栓形成和出血倾向模型分别评估了抗血栓形成和出血活性。FT-IR 和 NMR 表明 GaSP 是一种硫酸化琼脂。HPSEC 和元素微量分析分别显示出 284.8 kDa 的峰值摩尔质量和 0.63 的硫酸化程度。该分子将凝血时间延长了 2.1 倍，并抑制了 45%的血小板聚集。此外，它在 0.1、0.5 和 1 mg/kg 时显示出显著的剂量依赖性抗血栓形成作用，分别为 40%、64%和 80%，且无出血。这些结果表明 GaSP 具有有前途的抗血栓形成作用。
