Department of Chemistry, Graduate School of Science, Kyoto University, Kyoto 606-8502, Japan.
Phys Chem Chem Phys. 2011 Oct 14;13(38):16928-40. doi: 10.1039/c1cp21868a. Epub 2011 Sep 5.
A recently developed method to monitor reaction kinetics of intermolecular interaction is presented in this perspective. This method is based on time-dependent diffusion coefficient measurements using the pulsed laser induced transient grating technique. Using this method, time dependent biomolecular interactions, such as transient association and dissociation reactions in solution, have been successfully detected in real time. The principles and particular applications are described. In particular, unique features of this time-dependent diffusion coefficient method are emphasized by comparison with other techniques.
本文介绍了一种新的监测分子间相互作用反应动力学的方法。该方法基于脉冲激光诱导瞬态光栅技术的时间相关扩散系数测量。利用该方法，可以实时检测溶液中生物分子的瞬态结合和解离反应等时间相关的生物分子相互作用。文中描述了该方法的原理和具体应用。特别强调了与其他技术相比，该时间相关扩散系数方法的独特性。
