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杨凌糖多孢菌,一种从黄瓜植物中分离得到的具有拮抗作用的内生放线菌。

Saccharothrix yanglingensis sp. nov., an antagonistic endophytic actinomycete isolated from cucumber plant.

机构信息

State Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A&F University, Yangling, Shaanxi, China.

出版信息

Antonie Van Leeuwenhoek. 2012 Jan;101(1):141-6. doi: 10.1007/s10482-011-9631-9. Epub 2011 Sep 3.

Abstract

An endophytic actinomycete strain, designated Hhs.015(T), was isolated from roots of cucumber seedlings. The endophytic isolate was identified by means of a polyphasic taxonomic approach. On the basis of 16S rRNA gene sequence similarities, strain Hhs.015(T) was closely related to members of the genus Saccharothrix. DNA-DNA hybridization with the four closest relatives, Saccharothrix longispora NRRL B-16116(T), Saccharothrix xinjiangensis NRRL B-24321(T), Saccharothrix autraliensis CGMCC 4.1355(T) and Saccharothrix espanaensis CGMCC 4.1714(T), gave similarity values of 33.8, 28.2, 44.1 and 29.5%, respectively, which indicated that strain Hhs.015(T) represents a novel species of the genus Saccharothrix. This is consistent with the morphological, physiological and chemotaxonomic data. As a whole, these results suggest that strain Hhs.015(T) represents a novel Saccharothrix species. The name Saccharothrix yanglingensis sp. nov. is proposed, with the type strain Hhs.015(T) (=CGMCC 4.5627(T) = KCTC 19722(T)).

摘要

一株内生放线菌菌株,命名为 Hhs.015(T),从黄瓜幼苗的根部分离得到。该内生分离株通过多相分类学方法进行鉴定。基于 16S rRNA 基因序列相似性,菌株 Hhs.015(T)与 Saccharothrix 属的成员密切相关。与最接近的四个亲缘种,Saccharothrix longispora NRRL B-16116(T)、Saccharothrix xinjiangensis NRRL B-24321(T)、Saccharothrix autraliensis CGMCC 4.1355(T)和 Saccharothrix espanaensis CGMCC 4.1714(T)的 DNA-DNA 杂交相似度分别为 33.8%、28.2%、44.1%和 29.5%,表明菌株 Hhs.015(T)代表了 Saccharothrix 属的一个新种。这与形态学、生理学和化学分类学数据一致。总的来说,这些结果表明菌株 Hhs.015(T)代表了一种新的 Saccharothrix 种。提议使用新的 Saccharothrix yanglingensis sp. nov.名称,其模式菌株为 Hhs.015(T) (=CGMCC 4.5627(T) = KCTC 19722(T))。

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