The Key Laboratory of Biotechnology for Medicinal Plant of Jiangsu Province, School of Life Science, Jiangsu Normal University, Xuzhou, 221116, Jiangsu, People's Republic of China.
Antonie Van Leeuwenhoek. 2013 Feb;103(2):433-9. doi: 10.1007/s10482-012-9823-y. Epub 2012 Oct 5.
A novel actinomycete, designated strain KLBMP 1262(T), was isolated from a coastal plant Dendranthema indicum (Linn.) Des Moul collected from the coastal region of Nantong, Jiangsu Province, in east China and was studied in detail for its taxonomic position. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain KLBMP 1262(T) is a member of the genus Amycolatopsis. The 16S rRNA gene sequence similarity indicated that strain KLBMP 1262(T) is most closely related to Amycolatopsis sulphurea DSM 46092(T) (97.96 %), Amycolatopsis ultiminotia RP-AC36(T) (97.50 %) and Amycolatopsis jejuensis N7-3(T) (97.44 %); similarity to other type strains of the genus Amycolatopsis was less than 97.0 %. The organism was determined to have chemical and morphological features consistent with its classification in the genus Amycolatopsis such as meso-diaminopimelic acid as the diagnostic diamino acid in the cell wall peptidoglycan and arabinose and galactose as the diagnostic sugars. The predominant menaquinone was determined to be MK-9 (H(4)). The polar lipids detected were phosphatidylmethylethanolamine, phosphatidylethanolamine, an unknown aminophospholipid, two unknown glycolipids and several unknown lipids. The major fatty acids were found to be C(16:0), iso-C(16:0) and iso-C(15:0). DNA-DNA relatedness data, together with phenotypic differences, clearly distinguished the isolate from its closest relatives. On the basis of these phenotypic and genotypic data, the isolate is considered to represent a novel species, for which the name A. jiangsuensis sp. nov. is proposed. The type strain is KLBMP 1262(T) (=KCTC 19885 (T) = NBRC 108679(T)).
一株新的放线菌，菌株 KLBMP 1262(T)，从中国东部江苏省南通市沿海地区采集的一种植物菊属植物（Linn.）Des Moul 中分离出来，并对其分类地位进行了详细研究。基于 16S rRNA 基因序列的系统发育分析表明，菌株 KLBMP 1262(T)是游动放线菌属的一个成员。16S rRNA 基因序列相似性表明，菌株 KLBMP 1262(T)与硫链丝菌素放线菌 DSM 46092(T)（97.96%）、极限放线菌 RP-AC36(T)（97.50%）和济州放线菌 N7-3(T)（97.44%）最为密切相关；与游动放线菌属的其他模式菌株的相似性小于 97.0%。该生物被确定具有与游动放线菌属分类一致的化学和形态特征，例如细胞壁肽聚糖中的二氨基庚二酸为中二氨基庚二酸，阿拉伯糖和半乳糖为诊断糖。主要的menaquinone 被确定为 MK-9 (H(4))。检测到的极性脂质有磷脂酰甲醇乙醇胺、磷脂乙醇胺、一种未知的氨基磷脂、两种未知的糖脂和几种未知的脂质。主要脂肪酸为 C(16:0)、异 C(16:0)和异 C(15:0)。DNA-DNA 同源性数据以及表型差异清楚地区分了该分离株与其最接近的亲缘关系。基于这些表型和基因型数据，该分离株被认为代表一个新的种，建议命名为江苏游动放线菌。模式菌株为 KLBMP 1262(T)=KCTC 19885(T)=NBRC 108679(T)。
