Chalapathi Institute of Pharmaceutical Sciences, Lam, Guntur, Andhra Pradesh 522034, India.
Anal Bioanal Chem. 2011 Nov;401(8):2539-48. doi: 10.1007/s00216-011-5341-4. Epub 2011 Sep 3.
Authors developed a simple, sensitive, selective, rapid, rugged, and reproducible liquid chromatography-tandem mass spectrometry method for the quantification of eletriptan (EP) in human plasma using naratriptan (NP) as an internal standard (IS). Chromatographic separation was performed on Ascentis Express C18, 50 × 4.6 mm, 2.7 μm column. Mobile phase was composed of 0.1% formic acid: methanol (40:60 v/v), with 0.5 mL/min flow rate. Drug and IS were extracted by liquid-liquid extraction. EP and NP were detected with proton adducts at m/z 383.2→84.3 and 336.2→97.8 in multiple reaction monitoring (MRM) positive mode, respectively. The method was validated with the correlation coefficients of (r(2)) ≥ 0.9963 over a linear concentration range of 0.5-250.0 ng/mL. This method demonstrated intra- and inter-day precision within 1.4-9.2% and 4.4-5.5% and accuracy within 96.8-103% and 98.5-99.8% for EP. This method is successfully applied in the bioequivalence study of 24 human volunteers.
作者开发了一种简单、灵敏、选择性好、快速、稳健且重现性佳的液质联用法，用于人血浆中依来曲普坦（EP）的定量分析，内标物为那拉曲坦（NP）。色谱分离在 Ascentis Express C18 柱（50×4.6mm，2.7μm）上进行，流动相由 0.1%甲酸-甲醇（40:60，v/v）组成，流速为 0.5mL/min。采用液液萃取法提取药物和内标物。EP 和 NP 采用正离子多反应监测（MRM）模式，以质子加合物形式检测，m/z 分别为 383.2→84.3 和 336.2→97.8。该方法在 0.5-250.0ng/mL 的线性浓度范围内，相关系数（r(2)）均≥0.9963。该方法在 EP 分析中表现出日内和日间精密度分别为 1.4-9.2%和 4.4-5.5%，准确度分别为 96.8-103%和 98.5-99.8%。该方法成功应用于 24 名健康志愿者的生物等效性研究。
