Programa de Pós-Graduação em Neurociências, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brasil.
Metab Brain Dis. 2011 Dec;26(4):269-79. doi: 10.1007/s11011-011-9262-x. Epub 2011 Sep 3.
Type 1 diabetes mellitus (T1DM) is associated with neurocognitive dysfunction and astrogliosis. Physical exercise prevents cognitive impairments and induces important brain modifications. The aim of our study was to investigate the effect of treadmill exercise on spatial memory and astrocytic function in the hippocampus of a T1DM model. Fifty-seven Wistar rats were divided into four groups: trained control (TC) (n = 15), non-trained control (NTC) (n = 13), trained diabetic (TD) (n = 14) and non-trained diabetic (NTD) (n = 15). One month after streptozotocin-induced diabetes, exercise groups were submitted to 5 weeks of physical training, and then, all groups were assessed in the novel object-placement recognition task. Locomotor activity was analyzed in the open field apparatus using Any-maze software. The expression of glial fibrillary acidic protein (GFAP) and S100B in hippocampus and cerebrospinal fluid were measured using ELISA assay, and hippocampal GFAP immunoreactivity was evaluated by means of immunohistochemistry and optical densitometry. The results showed that physical exercise prevents and/or reverts spatial memory impairments observed in NTD animals (P < 0.01). Decreased locomotor activity was observed in both the NTD and TD groups when compared with controls (P < 0.05). ELISA and immunohistochemistry analyzes showed there was a reduction in GFAP levels in the hippocampus of NTD animals, which was not found in TD group. ELISA also showed an increase in S100B levels in the cerebrospinal fluid from the NTD group (P < 0.01) and no such increase was found in the TD group. Our findings indicate that physical exercise prevents and/or reverts the cognitive deficits and astroglial alterations induced by T1DM.
1 型糖尿病（T1DM）与神经认知功能障碍和星形胶质细胞增生有关。体育锻炼可预防认知障碍并引起重要的大脑改变。我们的研究目的是研究跑步机运动对 T1DM 模型中海马空间记忆和星形胶质细胞功能的影响。将 57 只 Wistar 大鼠分为四组：训练对照组（TC）（n = 15）、非训练对照组（NTC）（n = 13）、训练糖尿病组（TD）（n = 14）和非训练糖尿病组（NTD）（n = 15）。链脲佐菌素诱导糖尿病后 1 个月，运动组进行 5 周的体育锻炼，然后所有组均在新物体识别任务中进行评估。使用 Any-maze 软件在旷场装置中分析运动活性。使用 ELISA 测定试剂盒测量海马中神经胶质纤维酸性蛋白（GFAP）和 S100B 的表达，并通过免疫组织化学和光密度测定法评估海马 GFAP 免疫反应性。结果表明，体育锻炼可预防和/或逆转 NTD 动物观察到的空间记忆障碍（P < 0.01）。与对照组相比，NTD 和 TD 组的运动活性均降低（P < 0.05）。ELISA 和免疫组织化学分析显示，NTD 动物海马中的 GFAP 水平降低，而 TD 组中则未发现。ELISA 还显示 NTD 组脑脊液中 S100B 水平升高（P < 0.01），而 TD 组中未发现这种升高。我们的研究结果表明，体育锻炼可预防和/或逆转 T1DM 引起的认知缺陷和星形胶质细胞改变。
