Williams M E, Frierson H F, Tabbarah S, Ennis P S
Department of Internal Medicine, University of Virginia Health Sciences Center, Charlottesville 22908.
Am J Clin Pathol. 1990 Jun;93(6):754-9. doi: 10.1093/ajcp/93.6.754.
To determine the utility of Southern blot analysis for fine-needle aspiration samples, the authors prospectively analyzed immunoglobulin, T-cell receptor, c-myc, and bcl-2 gene rearrangements in 27 cases of known or suspected lymphoma. Adequate DNA for analysis was obtained from 20 of 27 cases (74%), including 18 of 22 (82%) with non-Hodgkin's lymphoma (NHL). Patients whose tumors showed sclerosis, cellular degeneration, or necrosis yielded inadequate DNA. Of the 18 NHLs with successful Southern blot studies, 17 tumors had a B-cell lineage and one was an adult T-cell leukemia/lymphoma; clonal integration of the human T-cell leukemia virus I (HTLV-I) genome was present in the latter case. Four cases had bcl-2 rearrangements and two had c-myc rearrangements. One patient with follicular small cleaved cell NHL that evolved to a small noncleaved cell NHL had coexisting bcl-2 and c-myc rearrangement in the aspiration specimen of the high-grade NHL, suggesting sequential bcl-2 and c-myc activation during the tumor's progression. Southern blot analysis is a useful technique for establishing tumor cell lineage, clonality, and the presence of oncogene rearrangements in fine-needle aspiration specimens of NHL.
为了确定Southern印迹分析在细针穿刺样本中的应用价值，作者前瞻性地分析了27例已知或疑似淋巴瘤病例中的免疫球蛋白、T细胞受体、c-myc和bcl-2基因重排情况。27例中有20例（74%）获得了足够用于分析的DNA，其中22例非霍奇金淋巴瘤（NHL）中有18例（82%）。肿瘤表现为硬化、细胞变性或坏死的患者所获得的DNA不足。在18例成功进行Southern印迹研究的NHL中，17例肿瘤为B细胞系，1例为成人T细胞白血病/淋巴瘤；后者存在人T细胞白血病病毒I（HTLV-I）基因组的克隆整合。4例有bcl-2重排，2例有c-myc重排。1例滤泡性小裂细胞NHL演变为小无裂细胞NHL的患者，在高级别NHL的穿刺标本中同时存在bcl-2和c-myc重排，提示在肿瘤进展过程中bcl-2和c-myc的激活具有先后顺序。Southern印迹分析是一种用于确定NHL细针穿刺标本中肿瘤细胞系、克隆性及癌基因重排情况的有用技术。
