Athan E, Foitl D R, Knowles D M
Department of Pathology, College of Physicians and Surgeons, Columbia University, New York, New York 10032.
Am J Pathol. 1991 Mar;138(3):591-9.
The authors investigated the structural organization of the bcl-1 locus, a putative oncogene associated with reciprocal chromosomal translocation t(11;14), by Southern blot hybridization analysis and its frequency, distribution, and prognostic significance in a panel of 156 clinically and pathologically well-defined B-cell chronic lymphocytic leukemias (CLLs) and non-Hodgkin's lymphomas (NHLs). The authors detected bcl-1 rearrangements in only 2 of 42 CLLs and 4 of 114 NHLs, specifically 3 of 29 diffuse small lymphocytic and 1 of 10 diffuse small cleaved cell and none of 5 diffuse intermediate lymphocytic, 13 follicular predominantly small cleaved, 17 follicular mixed small cleaved and large cell, 4 diffuse mixed small and large cell, 26 diffuse large cell, and 10 diffuse small noncleaved cell lymphomas. None of seven cases of Rai stage III or IV CLL or seven diffuse large cell lymphomas occurring as Richter's syndrome exhibited bcl-1 rearrangements. In conclusion, the bcl-1 locus rearranges in only about 4% of B-cell CLLs and NHLs, is predominantly rearranged in low-grade B-cell neoplasms, and does not appear to be preferentially associated with those occasional CLLs and low-grade NHLs displaying clinical aggressiveness, advanced clinical stage, or large cell transformation (Richter's syndrome). Therefore the demonstration of bcl-1 rearrangement does not appear to have clinically useful prognostic significance.
作者通过Southern印迹杂交分析研究了bcl-1基因座的结构组织，bcl-1是一种与相互染色体易位t(11;14)相关的假定癌基因，并研究了其在156例临床和病理特征明确的B细胞慢性淋巴细胞白血病（CLL）和非霍奇金淋巴瘤（NHL）中的频率、分布及预后意义。作者在42例CLL中的仅2例以及114例NHL中的4例中检测到bcl-1重排，具体为29例弥漫性小淋巴细胞淋巴瘤中的3例、10例弥漫性小裂细胞淋巴瘤中的1例，而5例弥漫性中等淋巴细胞淋巴瘤、13例滤泡性主要为小裂细胞淋巴瘤、17例滤泡性混合小裂细胞和大细胞淋巴瘤、4例弥漫性混合小和大细胞淋巴瘤、26例弥漫性大细胞淋巴瘤以及10例弥漫性小无裂细胞淋巴瘤中均未检测到。7例Rai III或IV期CLL或7例作为Richter综合征出现的弥漫性大细胞淋巴瘤中均未显示bcl-1重排。总之，bcl-1基因座仅在约4%的B细胞CLL和NHL中发生重排，主要在低度B细胞肿瘤中重排，并且似乎与那些偶尔表现出临床侵袭性、晚期临床分期或大细胞转化（Richter综合征）的CLL和低度NHL无优先关联。因此，bcl-1重排的检测似乎没有临床有用的预后意义。
