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里德-施特恩伯格细胞中c-myc和bcl-2癌基因产物的表达与爱泼斯坦-巴尔病毒的存在无关。

Expression of c-myc and bcl-2 oncogene products in Reed-Sternberg cells independent of presence of Epstein-Barr virus.

作者信息

Jiwa N M, Kanavaros P, van der Valk P, Walboomers J M, Horstman A, Vos W, Mullink H, Meijer C J

机构信息

Department of Pathology, Free University Hospital, Amsterdam, the Netherlands.

出版信息

J Clin Pathol. 1993 Mar;46(3):211-7. doi: 10.1136/jcp.46.3.211.

DOI:10.1136/jcp.46.3.211
PMID:8385158
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC501172/
Abstract

AIMS

To evaluate the expression of c-myc and bcl-2 oncogene products in Reed-Sternberg cells in Hodgkin's disease, especially in relation to Epstein-Barr virus infection and expression of EBV encoded latent membrane protein (LMP).

METHODS

Tissues from 33 cases of Hodgkin's disease were studied for the presence of EBV DNA by polymerase chain reaction (PCR) and DNA in situ hybridisation (DISH), for the presence of EBER-1 and EBER-2 EBV RNA by RNA in situ hybridisation (RISH); and for the presence of LMP, bcl-2, and c-myc proteins by immunohistochemical staining.

RESULTS

A substantial number of Reed-Sternberg cells expressed bcl-2 in 20 of 29 (69%) and c-myc in 30 of 32 (94%) Hodgkin's disease samples. In 18 of the 25 (72%) cases Reed-Sternberg cells expressed both oncogene products. Of these 18 cases, 10 (56%) were EBV-PCR positive; eight (44%) were EBV-PCR negative.

CONCLUSIONS

Reed-Sternberg cells in Hodgkin's disease frequently express both bcl-2 and c-myc oncogene products, suggesting that these oncogenes may act in concert in the pathogenesis of the disease. Moreover, the expression of c-myc and bcl-2 proteins in Reed-Sternberg cells is independent of EBV and LMP status.

摘要

目的

评估霍奇金病中里德-斯腾伯格细胞（Reed-Sternberg cell）中c-myc和bcl-2癌基因产物的表达，特别是与爱泼斯坦-巴尔病毒（Epstein-Barr virus，EBV）感染及EBV编码的潜伏膜蛋白（latent membrane protein，LMP）表达的关系。

方法

采用聚合酶链反应（PCR）和DNA原位杂交（DISH）研究33例霍奇金病组织中EBV DNA的存在情况，采用RNA原位杂交（RISH）研究EBER-1和EBER-2 EBV RNA的存在情况；采用免疫组织化学染色研究LMP、bcl-2和c-myc蛋白的存在情况。

结果

在29例霍奇金病样本中，有20例（69%）的大量里德-斯腾伯格细胞表达bcl-2，32例中有30例（94%）表达c-myc。在25例病例中的18例（72%）里德-斯腾伯格细胞同时表达这两种癌基因产物。在这18例病例中，10例（56%）EBV-PCR阳性；8例（44%）EBV-PCR阴性。

结论

霍奇金病中的里德-斯腾伯格细胞经常同时表达bcl-2和c-myc癌基因产物，提示这些癌基因可能在该疾病的发病机制中协同作用。此外，里德-斯腾伯格细胞中c-myc和bcl-2蛋白的表达与EBV和LMP状态无关。

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