Ricci Dante P, Silhavy Thomas J
Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA.
Biochim Biophys Acta. 2012 Apr;1818(4):1067-84. doi: 10.1016/j.bbamem.2011.08.020. Epub 2011 Aug 22.
The bacterial outer membrane (OM) is an exceptional biological structure with a unique composition that contributes significantly to the resiliency of Gram-negative bacteria. Since all OM components are synthesized in the cytosol, the cell must efficiently transport OM-specific lipids and proteins across the cell envelope and stably integrate them into a growing membrane. In this review, we discuss the challenges associated with these processes and detail the elegant solutions that cells have evolved to address the topological problem of OM biogenesis. Special attention will be paid to the Bam machine, a highly conserved multiprotein complex that facilitates OM β-barrel folding. This article is part of a Special Issue entitled: Protein Folding in Membranes.
细菌外膜（OM）是一种特殊的生物结构，具有独特的组成成分，对革兰氏阴性菌的抗逆性有重要贡献。由于所有外膜成分均在胞质溶胶中合成，细胞必须有效地将外膜特异性脂质和蛋白质转运穿过细胞膜，并将它们稳定地整合到生长中的膜中。在本综述中，我们讨论了与这些过程相关的挑战，并详细阐述了细胞为解决外膜生物发生的拓扑问题而进化出的精妙解决方案。我们将特别关注Bam机器，这是一种高度保守的多蛋白复合物，可促进外膜β桶的折叠。本文是名为“膜中蛋白质折叠”的特刊的一部分。
